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dc.contributor.author Badano, Maria Noel
dc.contributor.author Camicia, Gabriela Lorena
dc.contributor.author Lombardi, Maria Gabriela
dc.contributor.author Maglioco, Andrea Florencia
dc.contributor.author Cabrera, Gabriel Gustavo
dc.contributor.author Costa, Hector Luis
dc.contributor.author Meiss, Roberto Pablo
dc.contributor.author Piazzon, Margarita Isabel
dc.contributor.author Nepomnaschy, Irene
dc.date.available 2015-07-21T14:21:50Z
dc.date.issued 2013-04
dc.identifier.citation Badano, Maria Noel; Camicia, Gabriela Lorena; Lombardi, Maria Gabriela; Maglioco, Andrea Florencia; Cabrera, Gabriel Gustavo; et al.; B-cell lymphopoiesis is influenced by cathepsin L; Public Library Science; Plos One; 8; 4; 4-2013; e61347
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/11336/1314
dc.description.abstract Cathepsin L (CTSL) is a ubiquitously expressed lysosomal cysteine peptidase with diverse and highly specific functions. The involvement of CTSL in thymic CD4+ T-cell positive selection has been well documented. Using CTSLnkt/nkt mice that lack CTSL activity, we have previously demonstrated that the absence of CTSL activity affects the homeostasis of the T-cell pool by decreasing CD4+ cell thymic production and increasing CD8+ thymocyte production. Herein we investigated the influence of CTSL activity on the homeostasis of peripheral B-cell populations and bone marrow (BM) B-cell maturation. B-cell numbers were increased in lymph nodes (LN), spleen and blood from CTSLnkt/nkt mice. Increases in splenic B-cell numbers were restricted to transitional T1 and T2 cells and to the marginal zone (MZ) cell subpopulation. No alterations in the proliferative or apoptosis levels were detected in peripheral B-cell populations from CTSLnkt/nkt mice. In the BM, the percentage and the absolute number of pre-pro-B, pro-B, pre-B, immature and mature B cells were not altered. However, and experiments showed that BM B-cell production was markedly increased in CTSLnkt/nkt mice. Besides, BM B-cell emigration to the spleen was increased in CTSLnkt/nkt mice. Colony-forming unit pre-B (CFU pre-B) assays in the presence of BM stromal cells (SC) and reciprocal BM chimeras revealed that both BM B-cell precursors and SC would contribute to sustain the increased B-cell hematopoiesis in CTSLnkt/nkt mice. Overall, our data clearly demonstrate that CTSL negatively regulates BM B-cell production and output therefore influencing the homeostasis of peripheral B cells.
dc.format application/pdf
dc.language.iso eng
dc.publisher Public Library Science
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject Cathepsin L
dc.subject B cells
dc.subject lymphopoiesis
dc.subject.classification Inmunología
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title B-cell lymphopoiesis is influenced by cathepsin L
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2016-03-30 10:35:44.97925-03
dc.journal.volume 8
dc.journal.number 4
dc.journal.pagination e61347
dc.journal.pais Estados Unidos
dc.journal.ciudad San Francisco
dc.description.fil Fil: Badano, Maria Noel. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
dc.description.fil Fil: Camicia, Gabriela Lorena. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
dc.description.fil Fil: Lombardi, Maria Gabriela. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Centro de Estudios Farmacologicos y Botánicos; Argentina;
dc.description.fil Fil: Maglioco, Andrea Florencia. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
dc.description.fil Fil: Cabrera, Gabriel Gustavo. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
dc.description.fil Fil: Costa, Hector Luis. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
dc.description.fil Fil: Meiss, Roberto Pablo. Academia Nacional de Medicina de Buenos Aires; Argentina;
dc.description.fil Fil: Piazzon, Margarita Isabel. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
dc.description.fil Fil: Nepomnaschy, Irene. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
dc.journal.title Plos One
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://dx.doi.10.1371/journal.pone.0061347


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)