Mostrar el registro sencillo del ítem

dc.contributor.author
Badano, Maria Noel
dc.contributor.author
Camicia, Gabriela Lorena
dc.contributor.author
Lombardi, Maria Gabriela
dc.contributor.author
Maglioco, Andrea Florencia
dc.contributor.author
Cabrera, Gabriel Gustavo
dc.contributor.author
Costa, Hector Luis
dc.contributor.author
Meiss, Roberto Pablo
dc.contributor.author
Piazzon, Margarita Isabel
dc.contributor.author
Nepomnaschy, Irene
dc.date.available
2015-07-21T14:21:50Z
dc.date.issued
2013-04
dc.identifier.citation
Badano, Maria Noel; Camicia, Gabriela Lorena; Lombardi, Maria Gabriela; Maglioco, Andrea Florencia; Cabrera, Gabriel Gustavo; et al.; B-cell lymphopoiesis is influenced by cathepsin L; Public Library Science; Plos One; 8; 4; 4-2013; e61347
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/1314
dc.description.abstract
Cathepsin L (CTSL) is a ubiquitously expressed lysosomal cysteine peptidase with diverse and highly specific functions. The involvement of CTSL in thymic CD4+ T-cell positive selection has been well documented. Using CTSLnkt/nkt mice that lack CTSL activity, we have previously demonstrated that the absence of CTSL activity affects the homeostasis of the T-cell pool by decreasing CD4+ cell thymic production and increasing CD8+ thymocyte production. Herein we investigated the influence of CTSL activity on the homeostasis of peripheral B-cell populations and bone marrow (BM) B-cell maturation. B-cell numbers were increased in lymph nodes (LN), spleen and blood from CTSLnkt/nkt mice. Increases in splenic B-cell numbers were restricted to transitional T1 and T2 cells and to the marginal zone (MZ) cell subpopulation. No alterations in the proliferative or apoptosis levels were detected in peripheral B-cell populations from CTSLnkt/nkt mice. In the BM, the percentage and the absolute number of pre-pro-B, pro-B, pre-B, immature and mature B cells were not altered. However, and experiments showed that BM B-cell production was markedly increased in CTSLnkt/nkt mice. Besides, BM B-cell emigration to the spleen was increased in CTSLnkt/nkt mice. Colony-forming unit pre-B (CFU pre-B) assays in the presence of BM stromal cells (SC) and reciprocal BM chimeras revealed that both BM B-cell precursors and SC would contribute to sustain the increased B-cell hematopoiesis in CTSLnkt/nkt mice. Overall, our data clearly demonstrate that CTSL negatively regulates BM B-cell production and output therefore influencing the homeostasis of peripheral B cells.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cathepsin L
dc.subject
B Cells
dc.subject
Lymphopoiesis
dc.subject.classification
Inmunología
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
B-cell lymphopoiesis is influenced by cathepsin L
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
8
dc.journal.number
4
dc.journal.pagination
e61347
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Badano, Maria Noel. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
dc.description.fil
Fil: Camicia, Gabriela Lorena. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
dc.description.fil
Fil: Lombardi, Maria Gabriela. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Centro de Estudios Farmacologicos y Botánicos; Argentina;
dc.description.fil
Fil: Maglioco, Andrea Florencia. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
dc.description.fil
Fil: Cabrera, Gabriel Gustavo. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
dc.description.fil
Fil: Costa, Hector Luis. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
dc.description.fil
Fil: Meiss, Roberto Pablo. Academia Nacional de Medicina de Buenos Aires; Argentina;
dc.description.fil
Fil: Piazzon, Margarita Isabel. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
dc.description.fil
Fil: Nepomnaschy, Irene. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
dc.journal.title
Plos One
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.10.1371/journal.pone.0061347


Archivos asociados

 

Comunidades y colecciones

Mostrar el registro sencillo del ítem