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dc.contributor.author
Vazquez, Monica Hebe  
dc.contributor.author
Verón, Gustavo Luis  
dc.contributor.other
Skinner, Michael K.  
dc.date.available
2021-05-06T02:48:55Z  
dc.date.issued
2018  
dc.identifier.citation
Vazquez, Monica Hebe; Verón, Gustavo Luis; Laboratory evaluation of sperm-ovum interaction; Elsevier Inc; 5; 2018; 98-106  
dc.identifier.isbn
978-0-12-815145-7  
dc.identifier.uri
http://hdl.handle.net/11336/131443  
dc.description.abstract
The article will briefly address background information on relevant events involved in sperm–ovum interaction leading to fertilization. Reference to published work and laboratory protocols will be presented for the following assays: (1) the hyaluronan binding assay (evaluates sperm interaction with the cumulus cells extracellular matrix), (2) the hemizona assay and the sperm zona binding ratio assay (evaluate sperm interaction with the ZP), (3) the in vitro acrosome reaction (AR) tests ZIAR and ARIC (evaluate induced AR), and (4) the ZP-free hamster egg sperm penetration assay (evaluates several sperm functions, among them sperm fusion to the oolemma and sperm decondensation).  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Inc  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
LEYDIG CELLS  
dc.subject
SERTOLI CELLS  
dc.subject
SPERM  
dc.subject
ACROSOME  
dc.subject.classification
Biología Reproductiva  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Laboratory evaluation of sperm-ovum interaction  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.type
info:eu-repo/semantics/bookPart  
dc.type
info:ar-repo/semantics/parte de libro  
dc.date.updated
2020-08-24T18:08:30Z  
dc.journal.volume
5  
dc.journal.pagination
98-106  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina  
dc.description.fil
Fil: Verón, Gustavo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/B9780128012383648432  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/B978-0-12-801238-3.64843-2  
dc.conicet.paginas
3868  
dc.source.titulo
Encyclopedia of reproduction  
dc.conicet.nroedicion
2da.