Artículo
A new analogue of islet neogenesis associated protein with higher structural and plasma stability
Lynn, María Soledad
; Espinosa Silva, Yanis Ricardo
; Diambra, Luis Anibal
; Mccarthy, Andrés Norman; Liping, Liu; Ru, Bai; Román, Carolina Lisi
; Maiztegui, Barbara
; Flores, Luis Emilio
; Gagliardino, Juan Jose
Fecha de publicación:
01/2021
Editorial:
Adenine Press
Revista:
Journal Of Biomolecular Structure & Dynamics
ISSN:
0739-1102
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Islet Neogenesis Associated Protein pentadecapeptide (INGAP-PP) increases β-cell mass and function in experimental animals. A short clinical trial also yielded promising results. However, HTD4010, a new peptide derived from INGAP-PP, was developed in order to optimize its specific effects by minimizing its side effects. To study and compare the tertiary structure, stability dynamics, and plasma stability of HTD4010, an INGAP-PP analogue. Both peptides were pre-incubated in human, rat and mouse plasma at 37 °C, and their presence was identified and quantified by high performance liquid chromatography at different time-points. GROMACS 2019 package and the Gromos 54A7 force field were used to evaluate overall correlated motion of the peptide molecule during molecular dynamics simulation by essential dynamics. HTD4010 exhibited significantly larger plasma stability than INGAP-PP, and its structural stability was almost 3.36-fold higher than INGAP-PP. These results suggest that HTD4010 may facilitate longer tissue interaction, thereby developing higher potential biological effects. If so, HTD4010 may become a promising therapeutic agent to treat people with diabetes. Communicated by Ramaswamy H. Sarma.
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Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(CENEXA)
Articulos de CENTRO DE ENDOCRINOLOGIA EXP.Y APLICADA (I)
Articulos de CENTRO DE ENDOCRINOLOGIA EXP.Y APLICADA (I)
Citación
Lynn, María Soledad; Espinosa Silva, Yanis Ricardo; Diambra, Luis Anibal; Mccarthy, Andrés Norman; Liping, Liu; et al.; A new analogue of islet neogenesis associated protein with higher structural and plasma stability; Adenine Press; Journal Of Biomolecular Structure & Dynamics; 39; 3; 1-2021; 766-776
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