Evento
Post-implant biocompatibility of electro-spunded polycapronolactone nanocomposite fibrous scaffolds
Título:
Biocompatibilidad posimplante de matrices fibrosas nanocompuestas electrohiladas de policapronolactona
Missana, Liliana Raquel
; Moreno Garcia, Maria Florencia
; García, L. A.; Aimone, M.; Santiago, O.; Melián Salerno, Emanuel José; Camal Ruggieri, I. N.; Ramallo, M.; Rivero, Guadalupe
; Abraham, Gustavo Abel
; Feldman, Sara
Tipo del evento:
Congreso
Nombre del evento:
XXXVI Reunión Anual AAOMM
Fecha del evento:
30/10/2019
Institución Organizadora:
Asociación Argentina de Osteología y Metabolismo Mineral;
Título de la revista:
JBMR Plus
Editorial:
Wiley
e-ISSN:
2473-4039
Idioma:
Inglés
Clasificación temática:
Resumen
Tissue engineering aims regenerating de novo lost tissue. Our objective was to analyze the biocompatibility of the implant of PCL-nHAp, an electro-spun scaffold prepared by mixtures of a biocompatible and bioabsorbable polyester, poly(caprolactone), and a bioactive ceramic of nanofibrous structure (nanohydroxyapatite particles), in an in vivo model (New Zealand female rabbits). Scaffolds were 5 mm diameter PCL-nHAp discs, superficially modified to increase hydrophilicity by alkaline treatment and sterilized (peracetic acid). Treatments were (I) control, no bone lesion, (II) metaphyseal femoral bone lesion 3 mm deep and 5 mm diameter and PCL-nHAp implant; (III) lesion but no implant (n=6). Rabbit?s clinical evolution was adequate, without affecting gait. No differences in biochemical values (hemograms and transaminases) were detected after 1; 5 and 90 days. Histological studies (90days, 4n) showed that PCL-nHAp scaffold with a corrugated appearance, surrounded, from the outside inwards: A-Hematopoietic bone marrow with megakaryocytes and / or fat with congestive areas; B- few incipiently formed trabeculae, of varied forms;and C- presence of multinucleated giant cells, macrophages, lymphocytes combined with areas of hemorrhage and congestion. An adequate biocompatibility of the scaffolds can be observed, which in its imminent degradation process has not affected the biochemical parameters studied. A non-toxic and biologically active histological response is observed, with moderate levels of inflammation. We believe that longer post-implant periods will allow definitive conclusions.
Palabras clave:
biocompatibilidad
,
nanotecnologia
,
capronolactona
,
ingeniería de tejidos
Archivos asociados
Licencia
Identificadores
Colecciones
Eventos(CCT - ROSARIO)
Eventos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Eventos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Eventos(INTEMA)
Eventos de INST.DE INV.EN CIENCIA Y TECNOL.MATERIALES (I)
Eventos de INST.DE INV.EN CIENCIA Y TECNOL.MATERIALES (I)
Eventos(PROIMI)
Eventos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Eventos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Citación
Post-implant biocompatibility of electro-spunded polycapronolactone nanocomposite fibrous scaffolds; XXXVI Reunión Anual AAOMM; Ciudad Autónoma de Buenos Aires; Argentina; 2019; 14-15
Compartir