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dc.contributor.author
Alonso, Diego
dc.contributor.author
Serrano, Edgar
dc.contributor.author
Bermejo, Francisca J.
dc.contributor.author
Corral, Ricardo Santiago
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dc.date.available
2021-04-22T13:47:10Z
dc.date.issued
2019-01
dc.identifier.citation
Alonso, Diego; Serrano, Edgar; Bermejo, Francisca J.; Corral, Ricardo Santiago; HIF-1alpha-regulated MIF activation and Nox2-dependent ROS generation promote Leishmania amazonensis killing by macrophages under hypoxia; Academic Press Inc Elsevier Science; Cellular Immunology; 335; 1-2019; 15-21
dc.identifier.issn
0008-8749
dc.identifier.uri
http://hdl.handle.net/11336/130711
dc.description.abstract
Increasing attention is given to the finding that macrophages under hypoxia are capable of controlling infection by the intracellular protozoan parasite Leishmania amazonensis. The hypoxia-inducible factor (HIF)-1α has been shown to play an essential role in this enhanced innate immune response. Our study aimed to explore the HIF-1α-dependent mechanisms leading to reduced survival of the parasites residing in macrophages under low oxygen conditions. Hypoxia triggered (P < 0.01) NADPH oxidase 2 (Nox2) expression and reactive oxygen species (ROS) production in J774 macrophages upon 24-h infection with L. amazonensis. Furthermore, increased (P < 0.01) expression levels of HIF-1α and macrophage migration inhibitory factor (MIF) were detected in the infected cells grown at 3% oxygen tension. We found that either HIF-1α silencing, Nox2 inhibition or MIF antagonism caused a significant (P < 0.05) reversal of the improved leishmanicidal activity displayed by the hypoxic phagocytes. Taken together, our current results suggest that, under conditions of limited availability of oxygen, activation of the HIF-1α/MIF axis via Nox2/ROS induction promotes killing of L. amazonensis amastigotes by macrophages. Such protective mechanism might operate in L. amazonensis-infected tissues where low oxygen levels prevail.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Press Inc Elsevier Science
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
HYPOXIA
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HYPOXIA-INDUCIBLE FACTOR-1Α
dc.subject
LEISHMANIA AMAZONENSIS
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MACROPHAGE
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MACROPHAGE MIGRATION INHIBITORY FACTOR
dc.subject
REACTIVE OXYGEN SPECIES
dc.subject.classification
Inmunología
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
HIF-1alpha-regulated MIF activation and Nox2-dependent ROS generation promote Leishmania amazonensis killing by macrophages under hypoxia
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-11-19T21:32:25Z
dc.journal.volume
335
dc.journal.pagination
15-21
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Alonso, Diego. Universidad Nacional de Trujillo.; Perú
dc.description.fil
Fil: Serrano, Edgar. Universidad Nacional de Trujillo.; Perú
dc.description.fil
Fil: Bermejo, Francisca J.. Universidad Nacional de Trujillo.; Perú
dc.description.fil
Fil: Corral, Ricardo Santiago. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
dc.journal.title
Cellular Immunology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0008874918303125
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.cellimm.2018.10.007
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