Artículo
Photodynamic activity of Tagetes minuta extracts against superficial fungal infections
Giacone, Lucía; Cordisco, Estefanía
; Garrido, María Clara; Petenatti, Elisa Margarita; Sortino, Maximiliano Andrés
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
11/2019
Editorial:
Taylor & Francis Ltd
Revista:
Medical Mycology
ISSN:
1369-3786
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Candida and dermatophyte species are the most common causes of superficialmycoses because their treatment can be difficult due to limitations of current antifungal drugs in terms of toxicity, bioavailability, interactions, narrow-spectrum activity, and development of resistance. Photodynamic therapy (PDT) involves the topical administration of a photosensitizer in combination with light of an appropriate wavelength and molecular oxygen that produces reactive oxygen species (ROS), which promote damage to several vital components of the microorganism. Tagetes species are known as a source of thiophenes, biologically active compounds whose antifungal activity is enhanced by irradiation with UVA. The present investigation evaluated Tagetes minuta extracts as a photosensitizer on growth of Candida and dermatophytes and their effect on Candida virulence factors. T. minuta root hexane and dichloromethane extracts demonstrated high photodynamic antifungal activity. Bioautographic assays and chromatographic analysis revealed the presence of five thiophenes with reported photodynamic antifungal activities under UVA. Analysis of ROS production indicated that both type I and II reactions were involved in the activity of the extracts. In addition, the extracts inhibited virulence factors of Candida, such as adherence to epithelial surfaces and germ tube formation and showed efficacy against different Candida morphologies: Budding cells, cells with germ tube and biofilms. Results suggested that PDT with T. minuta extracts might become a valuable alternative to the already established antifungal drugs for the treatment of superficial fungal infections.
Palabras clave:
BIOFILM
,
PHOTODYNAMIC ANTIFUNGAL ACTIVITY
,
TAGETES MINUTA
,
VIRULENCE FACTORS
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - ROSARIO)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Citación
Giacone, Lucía; Cordisco, Estefanía; Garrido, María Clara; Petenatti, Elisa Margarita; Sortino, Maximiliano Andrés; Photodynamic activity of Tagetes minuta extracts against superficial fungal infections; Taylor & Francis Ltd; Medical Mycology; 58; 6; 11-2019; 797-809
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