Artículo
Disruption of antigenic variation is crucial for effective parasite vaccine
Rivero, Fernando David
; Saura, Alicia
; Prucca, Cesar German
; Carranza, Pedro Gabriel
; Torri, Alessandro
; Lujan, Hugo Daniel
Fecha de publicación:
05/2010
Editorial:
Nature Publishing Group
Revista:
Nature Medicine
ISSN:
1078-8956
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Giardia lamblia is a human intestinal pathogen. Like many protozoan microorganisms, Giardia undergoes antigenic variation, a mechanism assumed to allow parasites to evade the host's immune response, producing chronic and/or recurrent infections. Recently, we found that the mechanism controlling variant-specific surface protein (VSP) switching in Giardia involves components of the RNA interference machinery and that disruption of this pathway generates trophozoites simultaneously expressing many VSPs. Here we use these altered trophozoites to determine the role of antigenic variation in a gerbil model of giardiasis. Our results show that either primary infection with trophozoites simultaneously expressing many VSPs or immunization with purified VSPs from the transgenic cells protects gerbils from subsequent Giardia infections. These results constitute, to our knowledge, the first experimental evidence that antigenic variation is essential for parasite survival within hosts and that artificial disruption of this mechanism might be useful in generating vaccines against major pathogens that show similar behavior.
Palabras clave:
VACCINE
,
ANTIGENIC VARIATION
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Citación
Rivero, Fernando David; Saura, Alicia; Prucca, Cesar German; Carranza, Pedro Gabriel; Torri, Alessandro; et al.; Disruption of antigenic variation is crucial for effective parasite vaccine; Nature Publishing Group; Nature Medicine; 16; 5; 5-2010; 551-557
Compartir
Altmétricas