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dc.contributor.author
Tavares, Carolina Pereira  
dc.contributor.author
Vernal, Javier  
dc.contributor.author
Delena, Ricardo Alexandre  
dc.contributor.author
Lamattina, Lorenzo  
dc.contributor.author
Cassia, Raul Oscar  
dc.contributor.author
Terenzi, Hernán  
dc.date.available
2017-02-15T15:20:44Z  
dc.date.issued
2014-04  
dc.identifier.citation
Tavares, Carolina Pereira; Vernal, Javier; Delena, Ricardo Alexandre; Lamattina, Lorenzo; Cassia, Raul Oscar; et al.; S-nitrosylation influences the structure and DNA binding activity of AtMYB30 transcription factor from Arabidopsis thaliana; Elsevier Science; Biochimica Et Biophysica Acta-proteins And Proteomics; 1844; 4; 4-2014; 810-817  
dc.identifier.issn
1570-9639  
dc.identifier.uri
http://hdl.handle.net/11336/13045  
dc.description.abstract
MYB proteins are a family of transcription factors that play an important role in plant development and regulatory defense processes. Arabidopsis thaliana MYB30 (AtMYB30), a member of this protein family, is involved in cell death processes during the hypersensitive response (HR) of plants. HR is characterized by a vast production of reactive oxygen species (ROS) and nitric oxide (NO). NO may thus influence the binding of AtMYB30 to DNA. In this work we evaluated the effect of NO on AtMYB30 DNA binding activity, and also in the protein structural properties. A fully active minimal DNA-binding domain (DBD) of AtMYB30 (residues 11–116) containing two cysteine residues (C49 and C53) was overexpressed and purified. Site-directed mutagenesis was used to obtain AtMYB30 DBD mutants C49A and C53A. The DNA binding activity of AtMYB30 DBD, and Cys single mutants is clearly inhibited upon incubation with a NO donor, and S-nitrosylation was confirmed by the biotin switch assay. Finally, in order to understand the mechanism of NO effect on AtMYB30 DNA binding activity we performed circular dichroism analysis, to correlate the observed protein function inhibition and a potential structural impairment on AtMYB30 DBD. Indeed, NO modification of C49 and C53 residues promotes a subtle modification on the secondary structure of this transcription factor. We thus demonstrated, using various techniques, the in vitro effect of NO on AtMYB30 DBD, and thus the potential consequences of NO activity on plant metabolism influenced by this transcription factor.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/  
dc.subject
Myb Transcription Factors  
dc.subject
Nitric Oxide  
dc.subject
Nitrosylation  
dc.subject
Atmyb30 Dbd  
dc.subject
Arabidopsis  
dc.subject
Biotin Switch Assay  
dc.subject.classification
Bioquímica y Biología Molecular  
dc.subject.classification
Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
S-nitrosylation influences the structure and DNA binding activity of AtMYB30 transcription factor from Arabidopsis thaliana  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2017-02-10T18:13:37Z  
dc.journal.volume
1844  
dc.journal.number
4  
dc.journal.pagination
810-817  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Ámsterdam  
dc.description.fil
Fil: Tavares, Carolina Pereira. Universidade Federal Da Santa Catarina; Brasil  
dc.description.fil
Fil: Vernal, Javier. Universidade Federal Da Santa Catarina; Brasil  
dc.description.fil
Fil: Delena, Ricardo Alexandre. Universidade Federal Da Santa Catarina; Brasil  
dc.description.fil
Fil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina  
dc.description.fil
Fil: Cassia, Raul Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina  
dc.description.fil
Fil: Terenzi, Hernán. Universidade Federal Da Santa Catarina; Brasil  
dc.journal.title
Biochimica Et Biophysica Acta-proteins And Proteomics  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1570963914000429  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bbapap.2014.02.015