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dc.contributor.author
Tavares, Carolina Pereira
dc.contributor.author
Vernal, Javier
dc.contributor.author
Delena, Ricardo Alexandre
dc.contributor.author
Lamattina, Lorenzo
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dc.contributor.author
Cassia, Raul Oscar
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dc.contributor.author
Terenzi, Hernán
dc.date.available
2017-02-15T15:20:44Z
dc.date.issued
2014-04
dc.identifier.citation
Tavares, Carolina Pereira; Vernal, Javier; Delena, Ricardo Alexandre; Lamattina, Lorenzo; Cassia, Raul Oscar; et al.; S-nitrosylation influences the structure and DNA binding activity of AtMYB30 transcription factor from Arabidopsis thaliana; Elsevier Science; Biochimica Et Biophysica Acta-proteins And Proteomics; 1844; 4; 4-2014; 810-817
dc.identifier.issn
1570-9639
dc.identifier.uri
http://hdl.handle.net/11336/13045
dc.description.abstract
MYB proteins are a family of transcription factors that play an important role in plant development and regulatory defense processes. Arabidopsis thaliana MYB30 (AtMYB30), a member of this protein family, is involved in cell death processes during the hypersensitive response (HR) of plants. HR is characterized by a vast production of reactive oxygen species (ROS) and nitric oxide (NO). NO may thus influence the binding of AtMYB30 to DNA. In this work we evaluated the effect of NO on AtMYB30 DNA binding activity, and also in the protein structural properties. A fully active minimal DNA-binding domain (DBD) of AtMYB30 (residues 11–116) containing two cysteine residues (C49 and C53) was overexpressed and purified. Site-directed mutagenesis was used to obtain AtMYB30 DBD mutants C49A and C53A. The DNA binding activity of AtMYB30 DBD, and Cys single mutants is clearly inhibited upon incubation with a NO donor, and S-nitrosylation was confirmed by the biotin switch assay. Finally, in order to understand the mechanism of NO effect on AtMYB30 DNA binding activity we performed circular dichroism analysis, to correlate the observed protein function inhibition and a potential structural impairment on AtMYB30 DBD. Indeed, NO modification of C49 and C53 residues promotes a subtle modification on the secondary structure of this transcription factor. We thus demonstrated, using various techniques, the in vitro effect of NO on AtMYB30 DBD, and thus the potential consequences of NO activity on plant metabolism influenced by this transcription factor.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Myb Transcription Factors
dc.subject
Nitric Oxide
dc.subject
Nitrosylation
dc.subject
Atmyb30 Dbd
dc.subject
Arabidopsis
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Biotin Switch Assay
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
S-nitrosylation influences the structure and DNA binding activity of AtMYB30 transcription factor from Arabidopsis thaliana
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-02-10T18:13:37Z
dc.journal.volume
1844
dc.journal.number
4
dc.journal.pagination
810-817
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Ámsterdam
dc.description.fil
Fil: Tavares, Carolina Pereira. Universidade Federal Da Santa Catarina; Brasil
dc.description.fil
Fil: Vernal, Javier. Universidade Federal Da Santa Catarina; Brasil
dc.description.fil
Fil: Delena, Ricardo Alexandre. Universidade Federal Da Santa Catarina; Brasil
dc.description.fil
Fil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
dc.description.fil
Fil: Cassia, Raul Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
dc.description.fil
Fil: Terenzi, Hernán. Universidade Federal Da Santa Catarina; Brasil
dc.journal.title
Biochimica Et Biophysica Acta-proteins And Proteomics
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1570963914000429
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bbapap.2014.02.015
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