Mostrar el registro sencillo del ítem
dc.contributor.author
Zhou, Binghui
dc.contributor.author
Albarracín, Leonardo Miguel
dc.contributor.author
Indo, Yuhki
dc.contributor.author
Arce, Lorena Paola
dc.contributor.author
Masumizu, Yuki
dc.contributor.author
Tomokiyo, Mikado
dc.contributor.author
Islam, Md Aminul
dc.contributor.author
Garcia Castillo, Valeria
dc.contributor.author
Ikeda Ohtsubo, Wakako
dc.contributor.author
Nochi, Tomonori
dc.contributor.author
Morita, Hidetoshi
dc.contributor.author
Takahashi, Hideki
dc.contributor.author
Kurata, Shoichiro
dc.contributor.author
Villena, Julio Cesar
dc.contributor.author
Kitazawa, Haruki
dc.date.available
2021-04-19T13:25:01Z
dc.date.issued
2020-11
dc.identifier.citation
Zhou, Binghui; Albarracín, Leonardo Miguel; Indo, Yuhki; Arce, Lorena Paola; Masumizu, Yuki; et al.; Selection of immunobiotic ligilactobacillus salivarius strains from the intestinal tract of wakame-fed pigs: Functional and genomic studies; MDPI AG; Microorganisms; 8; 11; 11-2020; 1-28;1659
dc.identifier.issn
2076-2607
dc.identifier.uri
http://hdl.handle.net/11336/130333
dc.description.abstract
In this article, Ligilactobacillus salivarius FFIG strains, isolated from the intestinal tract of wakame-fed pigs, are characterized according to their potential probiotic properties. Strains were evaluated by studying their interaction with porcine intestinal epithelial (PIE) cells in terms of their ability to regulate toll-like receptor (TLR)-3- or TLR4-mediated innate immune responses, as well as by assessing their adhesion capabilities to porcine epithelial cells and mucins. These functional studies were complemented with comparative genomic evaluations using the complete genome sequences of porcine L. salivarius strains selected from subgroups that demonstrated different ‘immune’ and ‘adhesion’ phenotypes. We found that their immunomodulatory and adhesion capabilities are a strain-dependent characteristic. Our analysis indicated that the differential immunomodulatory and adhesive activities of FFIG strains would be dependent on the combination of several surface structures acting simultaneously, which include peptidoglycan, exopolysaccharides, lipoteichoic acid, and adhesins. Of note, our results indicate that there is no correlation between the immunomodulatory capacity of the strains with their adhesion ability to mucins and epithelial cells. Therefore, in the selection of strains destined to colonize the intestinal mucosa and modulate the immunity of the host, both properties must be adequately evaluated. Interestingly, we showed that L. salivarius FFIG58 functionally modulated the innate immune responses triggered by TLR3 and TLR4 activation in PIE cells and efficiently adhered to these cells. Moreover, the FFIG58 strain was capable of reducing rotavirus replication in PIE cells. Therefore, L. salivarius FFIG58 is a good candidate for further in vivo studying the protective effect of lactobacilli against intestinal infections in the porcine host. We also reported and analyzed, for the first time, the complete genome of several L. salivarius strains that were isolated from the intestine of pigs after the selective pressure of feeding the animals with wakame. Further genomic analysis could be of value to reveal the metabolic characteristics and potential of the FFIG strains in general and of the FFIG58 strain, in particular, relating to wakame by-products assimilation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
MDPI AG
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
ADHESION
dc.subject
GENOMICS
dc.subject
IMMUNOMODULATION
dc.subject
LIGILACTOBACILLUS SALIVARIUS
dc.subject
PIGS
dc.subject
PORCINE INTESTINAL EPITHELIAL CELLS
dc.subject
WAKAME
dc.subject.classification
Inmunología
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Selection of immunobiotic ligilactobacillus salivarius strains from the intestinal tract of wakame-fed pigs: Functional and genomic studies
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-01-18T20:58:08Z
dc.identifier.eissn
2076-2607
dc.journal.volume
8
dc.journal.number
11
dc.journal.pagination
1-28;1659
dc.journal.pais
Suiza
dc.journal.ciudad
Basilea
dc.description.fil
Fil: Zhou, Binghui. Tohoku University; Japón
dc.description.fil
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Ciencias de la Computación; Argentina. Tohoku University; Japón
dc.description.fil
Fil: Indo, Yuhki. Tohoku University; Japón
dc.description.fil
Fil: Arce, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Tohoku University; Japón
dc.description.fil
Fil: Masumizu, Yuki. Tohoku University; Japón
dc.description.fil
Fil: Tomokiyo, Mikado. Tohoku University; Japón
dc.description.fil
Fil: Islam, Md Aminul. Bangladesh Agricultural University; Bangladesh. Tohoku University; Japón
dc.description.fil
Fil: Garcia Castillo, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
dc.description.fil
Fil: Ikeda Ohtsubo, Wakako. Tohoku University; Japón
dc.description.fil
Fil: Nochi, Tomonori. Tohoku University; Japón
dc.description.fil
Fil: Morita, Hidetoshi. Okayama University; Japón
dc.description.fil
Fil: Takahashi, Hideki. Tohoku University; Japón
dc.description.fil
Fil: Kurata, Shoichiro. Tohoku University; Japón
dc.description.fil
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
dc.description.fil
Fil: Kitazawa, Haruki. Tohoku University; Japón
dc.journal.title
Microorganisms
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/microorganisms8111659
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/8/11/1659
Archivos asociados