Mostrar el registro sencillo del ítem
dc.contributor.author
Miles, Sebastián
dc.contributor.author
Portela, Madelón
dc.contributor.author
Cyrklaff, Marek
dc.contributor.author
Ancarola, María Eugenia
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Frischknecht, Friedrich
dc.contributor.author
Durán, Rosario
dc.contributor.author
Dematteis, Sylvia
dc.contributor.author
Mourglia Ettlin, Gustavo
dc.date.available
2021-04-16T11:59:42Z
dc.date.issued
2019-09
dc.identifier.citation
Miles, Sebastián; Portela, Madelón; Cyrklaff, Marek; Ancarola, María Eugenia; Frischknecht, Friedrich; et al.; Combining proteomics and bioinformatics to explore novel tegumental antigens as vaccine candidates against Echinococcus granulosus infection; Wiley-liss, div John Wiley & Sons Inc.; Journal of Cellular Biochemistry; 120; 9; 9-2019; 15320-15336
dc.identifier.issn
0730-2312
dc.identifier.uri
http://hdl.handle.net/11336/130178
dc.description.abstract
Echinococcus granulosus is the parasite responsible for cystic echinococcosis (CE), an important worldwide-distributed zoonosis. New effective vaccines against CE could potentially have great economic and health benefits. Here, we describe an innovative vaccine design scheme starting from an antigenic fraction enriched in tegumental antigens from the protoscolex stage (termed PSEx) already known to induce protection against CE. We first used mass spectrometry to characterize the protein composition of PSEx followed by Gene Ontology analysis to study the potential Biological Processes, Molecular Functions, and Cellular Localizations of the identified proteins. Following, antigenicity predictions and determination of conservancy degree against other organisms were determined. Thus, nine novel proteins were identified as potential vaccine candidates. Furthermore, linear B cell epitopes free of posttranslational modifications were predicted in the whole PSEx proteome through colocalization of in silico predicted epitopes within peptide fragments identified by matrix-assisted laser desorption/ionization-TOF/TOF. Resulting peptides were termed “clean linear B cell epitopes,” and through BLASTp scanning against all nonhelminth proteins, those with 100% identity against any other protein were discarded. Then, the secondary structure was predicted for peptides and their corresponding proteins. Peptides with highly similar secondary structure respect to their parental protein were selected, and those potentially toxic and/or allergenic were discarded. Finally, the selected clean linear B cell epitopes were mapped within their corresponding 3D-modeled protein to analyze their possible antibody accessibilities, resulting in 14 putative peptide vaccine candidates. We propose nine novel proteins and 14 peptides to be further tested as vaccine candidates against CE.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley-liss, div John Wiley & Sons Inc.
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ECHINOCOCCUS GRANULOSUS
dc.subject
IMMUNOINFORMATICS
dc.subject
LINEAR B CELL EPITOPES
dc.subject
MALDI-TOF/TOF
dc.subject
TEGUMENT
dc.subject
VACCINE
dc.subject.classification
Bioquímica y Biología Molecular
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
Ciencias Biológicas
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.title
Combining proteomics and bioinformatics to explore novel tegumental antigens as vaccine candidates against Echinococcus granulosus infection
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-12-18T19:32:30Z
dc.journal.volume
120
dc.journal.number
9
dc.journal.pagination
15320-15336
dc.journal.pais
Estados Unidos
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.description.fil
Fil: Miles, Sebastián. Universidad de la República; Uruguay
dc.description.fil
Fil: Portela, Madelón. Instituto Pasteur de Montevideo; Uruguay
dc.description.fil
Fil: Cyrklaff, Marek. Heidelberg University; Alemania
dc.description.fil
Fil: Ancarola, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
dc.description.fil
Fil: Frischknecht, Friedrich. Heidelberg University; Alemania
dc.description.fil
Fil: Durán, Rosario. Instituto Pasteur de Montevideo; Uruguay
dc.description.fil
Fil: Dematteis, Sylvia. Universidad de la República; Uruguay
dc.description.fil
Fil: Mourglia Ettlin, Gustavo. Universidad de la República; Uruguay
dc.journal.title
Journal of Cellular Biochemistry
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jcb.28799
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/jcb.28799
Archivos asociados