Evento
Understanding the mechanism of silver nanoparticle toxicity
Garces, Mariana Soledad; Magnani, Natalia Daniela
; Calabró López, María Valeria
; Marchini, Timoteo Oscar
; Caceres, Lourdes Catalina; Salgueiro, Jimena; Galdopórpora, Juan Manuel; Zubillaga, Marcela Beatriz
; Moretton, Marcela Analía
; Desimone, Martín Federico
; Pecorelli, A.; Alvarez, Silvia
; Valacchi, Guissepe; Evelson, Pablo Andrés
Tipo del evento:
Congreso
Nombre del evento:
Annual Meeting Society for Free Radical Research Europe
Fecha del evento:
19/06/2019
Institución Organizadora:
Society for Free Radical Research Europe;
Título de la revista:
Free Radical Biology and Medicine
Editorial:
Elsevier Science
ISSN:
0891-5849
Idioma:
Inglés
Clasificación temática:
Resumen
The development of silver nanoparticles (AgNP) applications increasedthe concern about their toxicity. As the respiratory system in one of theexposure routes, the aim of this study was to evaluate the AgNP harmfuleffects developed in lung. AgNP were able to cleave H2O2 leading to OHproduction. In A549 cells exposed to 2.5 μg/mL AgNP a decreasedmitochondrial basal and maximal respiration (p<0.01) were observedafter 3h, while an increased H2O2 production was observed after 1h(p<0.001), which in turn, increased the 4-HNE (p<0.01) and HO-1(p<0.01) levels after 24h. In an EpiAirway 3D tissue AgNP exposuredecreased the transepithelial electrical resistance (p<0.05). In vivo AgNPeffects were evaluated using Balb/c mice instilled with 0.1 mg AgNP/kgbody weight. Biodistribution evaluation showed the lung as the mainorgan of AgNP deposition, where 1h after AgNP exposure tissue O2consumption increased (p<0.05) due to increased NOX activity (p<0.05)and increased mitochondrial respiration (p<0.001) mainly by increasedcomplex I activity (p<0.01). Mitochondrial H2O2 production rateincreased (p<0.05) along with increased antioxidant enzymes activity(p<0.01) and decreased GSH/GSSG ratio. These results show that AgNPpresence in the lung may lead to oxidative damage and impairedepithelial function due to O2 metabolism alterations.
Palabras clave:
silver
,
nanoparticles
,
mitochondria
,
toxicity
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Eventos(IBIMOL)
Eventos de INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR
Eventos de INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR
Eventos(IQUIMEFA)
Eventos de INST.QUIMICA Y METABOLISMO DEL FARMACO (I)
Eventos de INST.QUIMICA Y METABOLISMO DEL FARMACO (I)
Citación
Understanding the mechanism of silver nanoparticle toxicity; Annual Meeting Society for Free Radical Research Europe; Ferrara; Italia; 2019; S25-S26
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