Capítulo de Libro
Metabolite Profiling by Direct Analysis in Real Time Mass Spectrometry
Título del libro: Mass Spectrometry in Metabolomics: Methods and Protocols
Jones, Christina M.; Monge, Maria Eugenia
; Fernández, Facundo M.
Otros responsables:
Raftery, Daniel
Fecha de publicación:
2014
Editorial:
Springer
ISBN:
978-1-4939-1257-5
Idioma:
Inglés
Clasificación temática:
Resumen
Untargeted metabolite profiling is a discovery tool in the identification of metabolites associated to the responses of perturbations to biological systems, such as a disease. Direct Analysis in Real Time mass spectrometry (DART MS) promises to be a powerful analytical technique for high-throughput metabolome analysis of human blood sera. Here, we describe the steps involved in untargeted blood sera metabolic profiling experiments using DART MS with two different sample introduction methods: probe mode and transmission mode geometries. Information regarding the optimization of different DART parameters that directly affect metabolite desorption and ionization, thus influencing sensitivity, is included.
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Capítulos de libros(CIBION)
Capítulos de libros de CENTRO DE INVESTIGACIONES EN BIONANOCIENCIAS "ELIZABETH JARES ERIJMAN"
Capítulos de libros de CENTRO DE INVESTIGACIONES EN BIONANOCIENCIAS "ELIZABETH JARES ERIJMAN"
Citación
Jones, Christina M.; Monge, Maria Eugenia; Fernández, Facundo M.; Metabolite Profiling by Direct Analysis in Real Time Mass Spectrometry; Springer; 1198; 2014; 275-289
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