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dc.contributor.author
Naojuk, Ortwin
dc.contributor.author
Francini, Flavio
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dc.contributor.author
Picton,Sally
dc.contributor.author
Jörns, Anne
dc.contributor.author
Bailey, Clifford J.
dc.contributor.author
Lenzen, Sigurd
dc.date.available
2021-04-08T13:18:56Z
dc.date.issued
2008-12
dc.identifier.citation
Naojuk, Ortwin; Francini, Flavio; Picton,Sally; Jörns, Anne; Bailey, Clifford J.; et al.; A new experimental protocol for preferential differentiation of mouse embryonic stem cells into insulin-producing cells; Cognizant Communication Corp; Cell Transplantation; 17; 10-11; 12-2008; 1231-1242
dc.identifier.issn
0963-6897
dc.identifier.uri
http://hdl.handle.net/11336/129608
dc.description.abstract
Mouse embiyonic stem (ES) cells have the potential to differentiate into insulin-producing cells, but efficient protocols for in vitro differentiation have not been established. Here we have developed a new optimized four-stage differentiation protocol and compared this with an established reference protocol. The new protocol minimized differentiation towards neuronal progeny, resulting in a population of insulin-producing cells with ß-cell characteristics but lacking neuronal features. The yield of glucagon and somatostatin cells was negligible. Crucial for this improved yield was the removal of a nestin selection step as well as removal of culture supplements that promote differentiation towards the neuronal lineage. Supplementation of the differentiation medium with insulin and fetal calf serum was beneficial for differentiation towards monohor-monal insulin-positive cells. After implantation into diabetic mice these insulin-producing cells produced a time-dependent improvement of the diabetic metabolic state, in contrast to cells differentiated according to the reference protocol. Using a spinner culture instead of an adherent culture of ES cells prevented the differentiation towards insulin-producing cells. Thus, prevention of cell attachment in a spinner culture represents a means to keep ES cells in an undifferentiated state and to inhibit differentiation. In conclusion, this study describes a new optimized four-stage protocol for differentiating ES cells to insulin-producing cells with minimal neuronal cell formation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Cognizant Communication Corp
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
DIABETES
dc.subject
DIFFERENTIATION
dc.subject
EMBRYONIC STEM CELLS
dc.subject
INSULIN
dc.subject.classification
Endocrinología y Metabolismo
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dc.subject.classification
Medicina Clínica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
A new experimental protocol for preferential differentiation of mouse embryonic stem cells into insulin-producing cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-05-11T15:18:18Z
dc.journal.volume
17
dc.journal.number
10-11
dc.journal.pagination
1231-1242
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Naojuk, Ortwin. Medizinische Hochschule Hannover; Alemania
dc.description.fil
Fil: Francini, Flavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina. Medizinische Hochschule Hannover; Alemania
dc.description.fil
Fil: Picton,Sally. Aston University. School of Life and Health Sciences; Reino Unido
dc.description.fil
Fil: Jörns, Anne. Medizinische Hochschule Hannover; Alemania
dc.description.fil
Fil: Bailey, Clifford J.. Aston University. School of Life and Health Sciences; Reino Unido
dc.description.fil
Fil: Lenzen, Sigurd. Medizinische Hochschule Hannover; Alemania
dc.journal.title
Cell Transplantation
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3727/096368908787236549
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.3727/096368908787236549
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