Evento
Effect of Hypertonic Saline Solution on cultures of human respiratory epithelium infected by respiratory syncytial virus
Figueroa, Juan Manuel; Primrose, Doris; Vdugour, Andrea; Ochoa, Valeria; Bitler, Julieta; Erra Diaz, Fernando Alberto
Tipo del evento:
Congreso
Nombre del evento:
ERS International Congress 2018
Fecha del evento:
09/2018
Institución Organizadora:
European Respiratory Society;
Título de la revista:
European Respiratory Society Journal
Editorial:
European Respiratory Society
ISSN:
0903-1936
e-ISSN:
1399-3003
Idioma:
Inglés
Clasificación temática:
Resumen
Introduction: nebulizations with hypertonic saline solution (HSS) has been incorporated in treatment of infant bronchiolitis but is currently a subject of controversy. Experimental studies on models of RSV infection have not been published.Objectives: to study the effect of HST in cultures of human respiratory epithelium infected with respiratory syncytial virus (RSV).Materials and Methods: Human respiratory epithelial cells (Hep-2 and Calu-3 lines) were cultured in monolayer. Cultures were divided into 3 groups: G1-control cells; G2-cells exposed to RSV for 3 hours, after which the culture medium was replaced by fresh medium without RSV for 72 hours; G3-cells exposed to RSV for 3 hours, after which culture medium was replaced by virus-free medium with addition of ClNA (final osmolarity, 400 m0sm) for 3 hs. Supernatant was stored and frozen for measurement of pro-inflammatory cytokines (IL-6, IL-8. ELISA). The percentage of RSV infected cells at 72 hours (flow cytometry) was determined in each group. The corresponding cell viability controls were performed (MTS-Cell Titer 96®), (Annexin V Apoptosis Detection Kit ?).Results: treatment with HSS did not alter the number of viable cells and reduced the percentage of Hep-2 and Calu-3 cells infected by RSV (68% and 40% respectively). This was associated with decrease in IL-6 and IL-8 release (p < 0.05).Conclusions: the addition of HSS decreased viral infection and pro-inflammatory response in cultures of human respiratory epithelial lines.
Palabras clave:
RSV
,
HYPERTONIC SALINE SOLUTION
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Identificadores
Colecciones
Eventos(INBIRS)
Eventos de INSTITUTO DE INVESTIGACIONES BIOMEDICAS EN RETROVIRUS Y SIDA
Eventos de INSTITUTO DE INVESTIGACIONES BIOMEDICAS EN RETROVIRUS Y SIDA
Eventos(SEDE CENTRAL)
Eventos de SEDE CENTRAL
Eventos de SEDE CENTRAL
Citación
Effect of Hypertonic Saline Solution on cultures of human respiratory epithelium infected by respiratory syncytial virus; ERS International Congress 2018; Paris; Francia; 2018; 1-1
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