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dc.contributor.author
Perez, Leonardo Martin
dc.contributor.author
Álvarez, B. L.
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Codony, F.
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Fittipaldi, M.
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Adrados, B.
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Peñuela Mesa, Gustavo Antonio
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Morató Farreras, Jordi
dc.date.available
2021-03-29T19:52:14Z
dc.date.issued
2010-09
dc.identifier.citation
Perez, Leonardo Martin; Álvarez, B. L.; Codony, F.; Fittipaldi, M.; Adrados, B.; et al.; A new microtitre plate screening method for evaluating the viability of aerobic respiring bacteria in high surface biofilms; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 51; 3; 9-2010; 331-337
dc.identifier.issn
0266-8254
dc.identifier.uri
http://hdl.handle.net/11336/129193
dc.description.abstract
Aims: It is difficult to determine the effects of bactericidal compounds against bacteria in a biofilm because classical procedures for determining cell viability require several working days, multiple complicated steps and are frequently only applicable to cells in suspension. We attempt to develop a compact, inexpensive and versatile system to measure directly the extent of biofilm formation from water systems and to determine the viability of respiring bacteria in high surface biofilms. Methods and Results: It has been reported that the reduction of tetrazolium sodium salts, such as XTT (sodium 3,3′-[1-[(phenylamino)carbonyl]-3,4-tetrazolium]Bis(4-methoxy)-6-nitro) benzene sulfonic acid hydrate), during active bacterial metabolism can be incorporated into a colorimetric method for quantifying cell viability. XTT is reduced to a soluble formazan compound during bacterial aerobic metabolism such that the amount of formazan generated is proportional to the bacterial biomass. Conclusions: We show here, for the first time, that this colorimetric approach can be used to determine the metabolic activity of adherent aerobic bacteria in a biofilm as a measure of cell viability. This technique has been used to estimate viability and proliferation of bacteria in suspension, but this is the first application to microbial communities in a real undisturbed biofilm. Significance and Impact of the Study: This simple new system can be used to evaluate the complex biofilm community without separating the bacteria from their support. Thus, the results obtained by this practice may be more representative of the circumstances in a natural system, opening the possibility to multiple potential applications.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BIOFILM(S)
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CYTOTOXICITY
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ENVIRONMENTAL
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MICROBIAL CONTAMINATION
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RAPID METHODS
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Otras Ciencias de la Tierra y relacionadas con el Medio Ambiente
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Ciencias de la Tierra y relacionadas con el Medio Ambiente
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CIENCIAS NATURALES Y EXACTAS
dc.title
A new microtitre plate screening method for evaluating the viability of aerobic respiring bacteria in high surface biofilms
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-02-18T15:22:25Z
dc.journal.volume
51
dc.journal.number
3
dc.journal.pagination
331-337
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Perez, Leonardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina. Universidad Politécnica de Catalunya; España
dc.description.fil
Fil: Álvarez, B. L.. Universidad Politécnica de Catalunya; España. Universidad de Antioquia; Colombia
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Fil: Codony, F.. Universidad Politécnica de Catalunya; España
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Fil: Fittipaldi, M.. Universidad Politécnica de Catalunya; España
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Fil: Adrados, B.. Universidad Politécnica de Catalunya; España
dc.description.fil
Fil: Peñuela Mesa, Gustavo Antonio. Universidad de Antioquia; Colombia
dc.description.fil
Fil: Morató Farreras, Jordi. Universidad Politécnica de Catalunya; España
dc.journal.title
Letters in Applied Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/j.1472-765X.2010.02902.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/j.1472-765X.2010.02902.x
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