Artículo
Efficient oral vaccination by bioengineering virus-like particles with protozoan surface proteins
Serradell, Marianela del Carmen.
; Rupil, Lucia
; Martino, Román Alejandro
; Prucca, Cesar German
; Carranza, Pedro Gabriel
; Saura, Alicia
; Fernandez, Elmer Andres
; Gargantini, Pablo Ruben
; Tenaglia, Albano Heraldo
; Petiti, Juan Pablo
; Tonelli, Renata R.; Reinoso Vizcaino, Nicolas Martin
; Echenique, Jose Ricardo
; Berod, Luciana; Piaggio, Eliane; Bellier, Bertrand; Sparwasser, Tim; Klatzmann, David; Lujan, Hugo Daniel
Fecha de publicación:
12/2019
Editorial:
Nature
Revista:
Nature Communications
e-ISSN:
2041-1723
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Intestinal and free-living protozoa, such as Giardia lamblia, express a dense coat of variant-specific surface proteins (VSPs) on trophozoites that protects the parasite inside the host’s intestine. Here we show that VSPs not only are resistant to proteolytic digestion and extreme pH and temperatures but also stimulate host innate immune responses in a TLR-4 dependent manner. We show that these properties can be exploited to both protect and adjuvant vaccine antigens for oral administration. Chimeric Virus-like Particles (VLPs) decorated with VSPs and expressing model surface antigens, such as influenza virus hemagglutinin (HA) and neuraminidase (NA), are protected from degradation and activate antigen presenting cells in vitro. Orally administered VSP-pseudotyped VLPs, but not plain VLPs, generate robust immune responses that protect mice from influenza infection and HA-expressing tumors. This versatile vaccine platform has the attributes to meet the ultimate challenge of generating safe, stable and efficient oral vaccines.
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Articulos (IMSATED)
Articulos de INSTITUTO MULTIDISCIPLINARIO DE SALUD, TECNOLOGIA Y DESARROLLO
Articulos de INSTITUTO MULTIDISCIPLINARIO DE SALUD, TECNOLOGIA Y DESARROLLO
Articulos(CIBICI)
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Articulos(CIDIE)
Articulos de CENTRO DE INV. Y DESARROLLO EN INMUNOLOGIA Y ENFERMEDADES INFECCIOSAS
Articulos de CENTRO DE INV. Y DESARROLLO EN INMUNOLOGIA Y ENFERMEDADES INFECCIOSAS
Articulos(CIQUIBIC)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Articulos(INICSA)
Articulos de INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Articulos de INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Citación
Serradell, Marianela del Carmen.; Rupil, Lucia; Martino, Román Alejandro; Prucca, Cesar German; Carranza, Pedro Gabriel; et al.; Efficient oral vaccination by bioengineering virus-like particles with protozoan surface proteins; Nature; Nature Communications; 10; 1; 12-2019; 361-380
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