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dc.contributor.author
Leung, Sherry See Wai
dc.contributor.author
Brewer, Jonathan
dc.contributor.author
Bagatolli, Luis Alberto
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dc.contributor.author
Thewalt, Jenifer L.
dc.date.available
2021-03-23T16:17:34Z
dc.date.issued
2019-12
dc.identifier.citation
Leung, Sherry See Wai; Brewer, Jonathan; Bagatolli, Luis Alberto; Thewalt, Jenifer L.; Measuring molecular order for lipid membrane phase studies: Linear relationship between Laurdan generalized polarization and deuterium NMR order parameter; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1861; 12; 12-2019; 1-8
dc.identifier.issn
0005-2736
dc.identifier.uri
http://hdl.handle.net/11336/128819
dc.description.abstract
Two dimensional phase separation in lipid membranes and cell membranes is of interest to biology because of the idea of membrane rafts — compositionally heterogeneous liquid crystal domains with cellular functions. Few quantitative tools exist for characterizing and differentiating coexisting phases on a molecular scale. Lipid acyl chain order can be measured directly using deuterium nuclear magnetic resonance spectroscopy (2H NMR), or inferred using fluorescence microscopy along with the environment-sensitive probe Laurdan. We found a linear relationship between the 2H NMR order parameter and Laurdan generalized polarization. This observed correlation supports the idea that lipid chain order is tightly associated with the amount and dynamics of water molecules at the glycerol backbone level of the membrane.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
DEUTERIUM NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY
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FLUORESCENCE MICROSCOPY
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LAURDAN
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LIPID MODEL MEMBRANE
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LIQUID DISORDERED-LIQUID ORDERED COEXISTENCE
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MEMBRANE ORDER
dc.subject.classification
Biofísica
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Measuring molecular order for lipid membrane phase studies: Linear relationship between Laurdan generalized polarization and deuterium NMR order parameter
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-11-19T21:19:12Z
dc.journal.volume
1861
dc.journal.number
12
dc.journal.pagination
1-8
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Leung, Sherry See Wai. University Fraser Simon. Faculty Of Applied Sciences; Canadá
dc.description.fil
Fil: Brewer, Jonathan. University Of Southern Denmark; Dinamarca
dc.description.fil
Fil: Bagatolli, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
dc.description.fil
Fil: Thewalt, Jenifer L.. University Fraser Simon. Faculty Of Applied Sciences; Canadá
dc.journal.title
Biochimica et Biophysica Acta - Biomembranes
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0005273619301993
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bbamem.2019.183053
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