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dc.contributor.author
Albertoni Borghese, Maria Florencia
dc.contributor.author
Bettini, Layne M.
dc.contributor.author
Nitta, Carlos H.
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de Frutos, Sergio
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Majowicz, Mónica
dc.contributor.author
González Bosc, Laura Veronica
dc.date.available
2017-02-10T19:29:44Z
dc.date.issued
2011-10
dc.identifier.citation
Albertoni Borghese, Maria Florencia; Bettini, Layne M.; Nitta, Carlos H.; de Frutos, Sergio; Majowicz, Mónica; et al.; Aquaporin-2 Promoter Is Synergistically Regulated by Nitric Oxide and Nuclear Factor of Activated T Cells.; Karger; Nephron EXTRA; 1; 1; 10-2011; 124-138
dc.identifier.issn
1664-5529
dc.identifier.uri
http://hdl.handle.net/11336/12873
dc.description.abstract
Background/aims: We have previously shown that aquaporin-2 (AQP2) is down-regulated in the renal medulla of rats made hypertensive by chronic inhibition of nitric oxide synthase (NOS). It has been shown that AQP2 expression is regulated by the calcineurin/ nuclear factor of activated T cells (NFATc). Nitric oxide (NO) regulates the activity of NFATc via c-Jun-N-terminal kinase 2 (JNK2). Therefore, we hypothesized that increases in NO enhance NFATc-mediated up-regulation of AQP2 promoter activity.
Methods: AQP2 mRNA and protein expression were detected in mouse renal papilla. AQP2-promoter-luciferase reporter and NFAT-luciferase reporter transfected MDCK cells were used to determine AQP2 promoter activity and NFATc activity, respectively. Cells were incubated with classic activators and inhibitors of NFATc and NO pathway.
Results: Our results demonstrate that both Ca2+ and NO have a synergistic effect in increasing AQP2 mRNA and protein in mouse papilla, and in activating the AQP2 promoter in kidney-derived cells. In addition, NO enhances Ca2+-induced NFATc activation. The underlying mechanism involves increased NFATc nuclear import and decreased export via PKG-mediated inhibition of JNK1/2.
Conclusions: This is the first study defining novel regulatory roles for NO and NFATc in the control of AQP2 expression, which is an important renal protein.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Karger
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Aquaporin 2
dc.subject
Nitric Oxide
dc.subject
Nfatc
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Aquaporin-2 Promoter Is Synergistically Regulated by Nitric Oxide and Nuclear Factor of Activated T Cells.
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-02-09T18:22:52Z
dc.journal.volume
1
dc.journal.number
1
dc.journal.pagination
124-138
dc.journal.pais
Suiza
dc.journal.ciudad
Basilea
dc.description.fil
Fil: Albertoni Borghese, Maria Florencia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
dc.description.fil
Fil: Bettini, Layne M.. University Of New Mexico. School Of Medicine. Departament Of Cell Biology And Phisiology; Estados Unidos
dc.description.fil
Fil: Nitta, Carlos H.. University Of New Mexico. School Of Medicine. Departament Of Cell Biology And Phisiology; Estados Unidos
dc.description.fil
Fil: de Frutos, Sergio. University Of New Mexico. School Of Medicine. Departament Of Cell Biology And Phisiology; Estados Unidos
dc.description.fil
Fil: Majowicz, Mónica. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Biología Celular y Molecular; Argentina
dc.description.fil
Fil: González Bosc, Laura Veronica. University Of New Mexico. School Of Medicine. Departament Of Cell Biology And Phisiology; Estados Unidos
dc.journal.title
Nephron EXTRA
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/FullText/333066
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3290856/
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1159/000333066
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