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dc.contributor.author
Bertuzzi, Mariana Lucía
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dc.contributor.author
Torres, Edita Yola
dc.contributor.author
Huanca, Teodosio
dc.contributor.author
Neild, Deborah
dc.contributor.author
Carretero, Maria Ignacia
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dc.date.available
2021-03-19T14:20:43Z
dc.date.issued
2020-11-30
dc.identifier.citation
Bertuzzi, Mariana Lucía; Torres, Edita Yola; Huanca, Teodosio; Neild, Deborah; Carretero, Maria Ignacia; Comparison of extenders with the addition of egg yolk for cooling alpaca sperm obtained from deferent ducts; Frontiers Media S.A.; Frontiers in Veterinary Science; 7; 597954; 30-11-2020; 1-9
dc.identifier.uri
http://hdl.handle.net/11336/128638
dc.description.abstract
The use of non-commercial and commercial extenders for cooling alpaca sperm has already been reported, the latter showing certain advantages over the first. The Andromed® (AM) extender was created for use in ruminants and has also been tested in ejaculated and epididymal alpaca sperm. According to the manufacturer, this extender does not need the addition of egg yolk (EY); however, it is known that the addition of EY to some extenders improves the preservation of cooled sperm. The objective of this study therefore was to compare a non-commercial extender (Tris) with the addition of EY vs. the commercial extender AM with and without the addition of EY, for cooling alpaca sperm obtained from diverted deferent ducts. Fifteen pools of deferent duct sperm were formed using samples from two or three different males for each. Each sperm pool was evaluated and then divided into three aliquots that were diluted to a final concentration of 30 × 106 sperm ml-1 (0 h) with either: (1) Tris with 20% EY (T-EY), (2) AM, or (3) AM with 20% EY (AM-EY). Samples were cooled to 5°C and the following sperm parameters were evaluated after 24 and 48 h of storage: motility, viability, membrane function, acrosome integrity, morphology, and chromatin condensation. Motility was also evaluated after 72 h of storage. The samples that best preserved progressive and total sperm motility at the 24 and 48 h evaluation periods were the ones diluted with AM-EY, observing that with this extender these motility patterns decreased significantly after 72 h of storage compared to time 0 h (p < 0.05). A significant decrease (p < 0.05) in total and progressive motility was observed at 48 h for the T-EY and AM extender compared to 0 h. AM was the only extender in which the percentages of viable sperm decreased significantly (p < 0.05) after 48 h of conservation. For the rest of sperm parameters evaluated, no significant differences were observed between any of the extenders at any evaluation time. The Andromed® extender with the addition of 20% EY could be an alternative option for cooling alpaca sperm obtained from deferent ducts.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Media S.A.
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
ALPACA
dc.subject
COOLING
dc.subject
DEFERENT DUCTS
dc.subject
EGG YOLK
dc.subject
EXTENDERS
dc.subject
SPERM
dc.subject.classification
Otras Ciencias Veterinarias
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dc.subject.classification
Ciencias Veterinarias
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dc.subject.classification
CIENCIAS AGRÍCOLAS
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dc.title
Comparison of extenders with the addition of egg yolk for cooling alpaca sperm obtained from deferent ducts
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-03-05T18:46:45Z
dc.identifier.eissn
2297-1769
dc.journal.volume
7
dc.journal.number
597954
dc.journal.pagination
1-9
dc.journal.pais
Suiza
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dc.journal.ciudad
Lausana
dc.description.fil
Fil: Bertuzzi, Mariana Lucía. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina
dc.description.fil
Fil: Torres, Edita Yola. Universidad Nacional del Altiplano Puno; Perú
dc.description.fil
Fil: Huanca, Teodosio. Instituto Nacional de Innovación Agraria. Centro de Investigación y Producción Quinsachata; Perú
dc.description.fil
Fil: Neild, Deborah. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
dc.description.fil
Fil: Carretero, Maria Ignacia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina
dc.journal.title
Frontiers in Veterinary Science
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fvets.2020.597954
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fvets.2020.597954/full
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/33330721/
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733988/
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