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dc.contributor.author
Panzetta, Maria Emilia
dc.contributor.author
Lujan, Agustin Leonardo
dc.contributor.author
Bastidas, Robert J.
dc.contributor.author
Damiani, Maria Teresa
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Valdivia, Raphael H.
dc.contributor.author
Saka, Hector Alex
dc.date.available
2021-03-17T15:26:09Z
dc.date.issued
2019-04
dc.identifier.citation
Panzetta, Maria Emilia; Lujan, Agustin Leonardo; Bastidas, Robert J.; Damiani, Maria Teresa; Valdivia, Raphael H.; et al.; Ptr/CTL0175 is required for the efficient recovery of chlamydia trachomatisfrom stress induced by gamma-interferon; Frontiers Media S.A.; Frontiers in Microbiology; 10; 4-2019; 1-18
dc.identifier.uri
http://hdl.handle.net/11336/128472
dc.description.abstract
Chlamydia trachomatis is the most common sexually transmitted bacterial pathogen in humans and a frequent cause of asymptomatic, persistent infections leading to serious complications, particularly in young women. Chlamydia displays a unique obligate intracellular lifestyle involving the infectious elementary body and the replicative reticulate body. In the presence of stressors such as gamma-interferon (IFNγ) or beta-lactam antibiotics, C. trachomatis undergoes an interruption in its replication cycle and enters a viable but non-cultivable state. Upon removal of the stressors, surviving C. trachomatis resume cell division and developmental transitions. In this report, we describe a genetic screen to identify C. trachomatis mutants with defects in recovery from IFNγ- and/or penicillin-induced stress and characterized a chemically derived C. trachomatis mutant strain that exhibited a significant decrease in recovery from IFNγ- but not penicillin-induced stress. Through lateral gene transfer and targeted insertional gene inactivation we identified ptr, encoding a predicted protease, as a gene required for recovery from IFNγ-induced stress. A C. trachomatis LGV-L2 ptr-null strain displayed reduced generation of infectious progeny and impaired genome replication upon removal of IFNγ. This defect was restored by introducing a wild type copy of ptr on a plasmid, indicating that Ptr is required for a rapid growth upon removal of IFN?. Ptr was expressed throughout the developmental cycle and localized to the inclusion lumen. Overall, our findings indicate that the putative secreted protease Ptr is required for C. trachomatis to specifically recover from IFNγ- but not penicillin-induced stress.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Media S.A.
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CHLAMYDIA TRACHOMATIS
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IFNΓ
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IFNΓ-INDUCED STRESS
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PENICILLIN
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PERSISTENCE
dc.subject
PTR
dc.subject.classification
Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Ptr/CTL0175 is required for the efficient recovery of chlamydia trachomatisfrom stress induced by gamma-interferon
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-11-18T16:43:02Z
dc.identifier.eissn
1664-302X
dc.journal.volume
10
dc.journal.pagination
1-18
dc.journal.pais
Suiza
dc.description.fil
Fil: Panzetta, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
dc.description.fil
Fil: Lujan, Agustin Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
dc.description.fil
Fil: Bastidas, Robert J.. University of Duke; Estados Unidos
dc.description.fil
Fil: Damiani, Maria Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
dc.description.fil
Fil: Valdivia, Raphael H.. University of Duke; Estados Unidos
dc.description.fil
Fil: Saka, Hector Alex. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
dc.journal.title
Frontiers in Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2019.00756/full
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fmicb.2019.00756
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