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dc.contributor.author
Porcari, Cintia Yamila  
dc.contributor.author
Araujo, Iracema Gomes  
dc.contributor.author
Urzedo-Rodrigues, Lilia  
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De Luca, Laurival Antonio  
dc.contributor.author
Menani, José Vanderlei  
dc.contributor.author
Caeiro, Ximena Elizabeth  
dc.contributor.author
Imboden, Hans  
dc.contributor.author
Antunes-Rodrigues, José  
dc.contributor.author
Reis, Luís Carlos  
dc.contributor.author
Vivas, Laura Marta  
dc.contributor.author
Godino, Maria Andrea del Milagro  
dc.contributor.author
Mecawi, Andre Souza  
dc.date.available
2021-03-17T15:09:48Z  
dc.date.issued
2019-04  
dc.identifier.citation
Porcari, Cintia Yamila; Araujo, Iracema Gomes; Urzedo-Rodrigues, Lilia; De Luca, Laurival Antonio; Menani, José Vanderlei; et al.; Whole body sodium depletion modifies AT1 mRNA expression and serotonin content in the dorsal raphe nucleus; Wiley Blackwell Publishing, Inc; Journal of Neuroendocrinology; 31; 4; 4-2019; 1-10  
dc.identifier.issn
0953-8194  
dc.identifier.uri
http://hdl.handle.net/11336/128459  
dc.description.abstract
Angiotensin II (Ang II) acts on Ang II type 1 (AT1) receptors located in the organum vasculosum and subfornical organ (SFO) of the lamina terminalis as a main facilitatory mechanism of sodium appetite. The brain serotonin (5-HT) system with soma located in the dorsal raphe nucleus (DRN) provides a main inhibitory mechanism. In the present study, we first investigated the existence of Ang II AT1 receptors in serotonergic DRN neurones. Then, we examined whether whole body sodium depletion affects the gene expression of the AT1a receptor subtype and the presumed functional significance of AT1 receptors. Using confocal microscopy, we found that tryptophan hydroxylase-2 and serotonin neurones express AT1 receptors in the DRN. Immunofluorescence quantification showed a significant reduction in 5-HT content but no change in AT1 receptor expression or AT1/5-HT colocalisation in the DRN after sodium depletion. Whole body sodium depletion also significantly increased Agtr1a mRNA expression in the SFO and DRN. Oral treatment with the AT1 receptor antagonist losartan reversed the changes in Agtr1a expression in the SFO but not the DRN. Losartan injection into either the DRN or the mesencephalic aqueduct had no influence on sodium depletion-induced 0.3 mol L -1 NaCl intake. The results indicate the expression of Agtr1a mRNA in the DRN and SFO as a marker of sodium depletion. They also suggest that serotonergic DRN neurones are targets for Ang II. However, the function of their AT1 receptors remains elusive.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Wiley Blackwell Publishing, Inc  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
ANGIOTENSIN II  
dc.subject
AT1 RECEPTOR  
dc.subject
DORSAL RAPHE NUCLEUS  
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SEROTONIN  
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SODIUM APPETITE  
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SUBFORNICAL ORGAN  
dc.subject.classification
Neurociencias  
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Medicina Básica  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Whole body sodium depletion modifies AT1 mRNA expression and serotonin content in the dorsal raphe nucleus  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2020-11-19T21:19:37Z  
dc.identifier.eissn
1365-2826  
dc.journal.volume
31  
dc.journal.number
4  
dc.journal.pagination
1-10  
dc.journal.pais
Reino Unido  
dc.journal.ciudad
Londres  
dc.description.fil
Fil: Porcari, Cintia Yamila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina  
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Fil: Araujo, Iracema Gomes. Universidade Federal Rural Do Rio de Janeiro; Brasil  
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Fil: Urzedo-Rodrigues, Lilia. Universidade Estadual Paulista; Brasil  
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Fil: De Luca, Laurival Antonio. Universidade Estadual Paulista; Brasil  
dc.description.fil
Fil: Menani, José Vanderlei. Universidade Estadual Paulista; Brasil  
dc.description.fil
Fil: Caeiro, Ximena Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina  
dc.description.fil
Fil: Imboden, Hans. University of Bern; Suiza  
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Fil: Antunes-Rodrigues, José. Universidade de Sao Paulo; Brasil  
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Fil: Reis, Luís Carlos. Universidade Federal Rural Do Rio de Janeiro; Brasil  
dc.description.fil
Fil: Vivas, Laura Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina  
dc.description.fil
Fil: Godino, Maria Andrea del Milagro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina  
dc.description.fil
Fil: Mecawi, Andre Souza. Universidade Federal de Sao Paulo; Brasil  
dc.journal.title
Journal of Neuroendocrinology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/jne.12703  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/jne.12703