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dc.contributor.author
Beckwith, Esteban Javier
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dc.contributor.author
Lelito, Katherine R.
dc.contributor.author
Hsu, Yun Wei A.
dc.contributor.author
Medina, Billie M.
dc.contributor.author
Shafer, Orie
dc.contributor.author
Ceriani, Maria Fernanda
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dc.contributor.author
de la Iglesia, Horacio O
dc.date.available
2017-02-09T21:44:23Z
dc.date.issued
2011-12
dc.identifier.citation
Beckwith, Esteban Javier; Lelito, Katherine R.; Hsu, Yun Wei A.; Medina, Billie M.; Shafer, Orie; et al.; Functional conservation of clock output signaling between flies and intertidal crabs; Sage Publications; Journal Of Biological Rhythms.; 26; 6; 12-2011; 518-529
dc.identifier.issn
0748-7304
dc.identifier.uri
http://hdl.handle.net/11336/12810
dc.description.abstract
Intertidal species have both circadian and circatidal clocks. Although the behavioral evidence for these oscillators is more than 5 decades old, virtually nothing is known about their molecular clockwork. Pigment-dispersing hormones (PDHs) were originally described in crustaceans. Their insect homologs, pigment-dispersing factors (PDFs), have a prominent role as clock output and synchronizing signals released from clock neurons. We show that gene duplication in crabs has led to two PDH genes (β-pdh-I and β-pdh-II). Phylogenetically, β-pdh-I is more closely related to insect pdf than to β-pdh-II, and we hypothesized that β-PDH-I may represent a canonical clock output signal. Accordingly, β-PDH-I expression in the brain of the intertidal crab Cancer productus is similar to that of PDF in Drosophila melanogaster, and neurons that express PDH-I also show CYCLE-like immunoreactivity. Using D. melanogaster pdf-null mutants (pdf(01)) as a heterologous system, we show that β-pdh-I is indistinguishable from pdf in its ability to rescue the mutant arrhythmic phenotype, but β-pdh-II fails to restore the wild-type phenotype. Application of the three peptides to explanted brains shows that PDF and β-PDH-I are equally effective in inducing the signal transduction cascade of the PDF receptor, but β-PDH-II fails to induce a normal cascade. Our results represent the first functional characterization of a putative molecular clock output in an intertidal species and may provide a critical step towards the characterization of molecular components of biological clocks in intertidal organisms.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Sage Publications
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Pdh
dc.subject
Pdf
dc.subject
Circatidal Clocks,
dc.subject
Intertidal Species
dc.subject.classification
Biología
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Functional conservation of clock output signaling between flies and intertidal crabs
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-02-07T13:52:05Z
dc.journal.volume
26
dc.journal.number
6
dc.journal.pagination
518-529
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Beckwith, Esteban Javier. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
dc.description.fil
Fil: Lelito, Katherine R.. University Of Michigan; Estados Unidos
dc.description.fil
Fil: Hsu, Yun Wei A.. University Of Washington; Estados Unidos
dc.description.fil
Fil: Medina, Billie M.. University Of Washington; Estados Unidos
dc.description.fil
Fil: Shafer, Orie. University Of Michigan; Estados Unidos
dc.description.fil
Fil: Ceriani, Maria Fernanda. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
dc.description.fil
Fil: de la Iglesia, Horacio O. University Of Washington; Estados Unidos
dc.journal.title
Journal Of Biological Rhythms.
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/pdf/10.1177/0748730411420242
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1177/0748730411420242
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