Artículo

Feasibility of using RFLP of PCR-amplified 16S rRNA gene(s) for rapid differentiation of isolates of aerobic spore-forming bacteria from honey

López, Ana ClaudiaIcon ; Alippi, Adriana Mónica
Fecha de publicación: 10/2019
Editorial: Elsevier Science
Revista: Journal of Microbiological Methods
ISSN: 0167-7012
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:

Resumen

This study aimed to assess the feasibility of using RFLP of PCR-amplified 16S rRNA gene (s) by using universalprimers 27f/1492r and a combination of three restriction enzymes, AluI, CfoI, and TaqI, for a low-cost, rapidscreen for a primarily differentiation of isolates of the complex of aerobic spore-forming bacteria commonlyfound in honey samples. The described method produced unique and distinguishable patterns to differentiateamong 80 isolates belonging to 26 different species of Bacillus, Brevibacillus, Lysinibacillus, Rummeliibacillus, andPaenibacillus reported in honey and other apiarian sources.
Palabras clave: PCR-RFLP , BACILLUS , BREVIBACILLUS , LYSINIBACILLUS
 
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/128080
URL: https://www.sciencedirect.com/science/article/abs/pii/S016770121930524X
DOI: http://dx.doi.org/10.1016/j.mimet.2019.105690
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Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Citación
López, Ana Claudia; Alippi, Adriana Mónica; Feasibility of using RFLP of PCR-amplified 16S rRNA gene(s) for rapid differentiation of isolates of aerobic spore-forming bacteria from honey; Elsevier Science; Journal of Microbiological Methods; 165; 10-2019; 1-11
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