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dc.contributor.author
Fait, María Elisa
dc.contributor.author
da Costa, Helen P. S.
dc.contributor.author
Freitas, Cleverson D. T.
dc.contributor.author
Bakas, Laura Susana
dc.contributor.author
Morcelle del Valle, Susana Raquel
dc.date.available
2021-03-10T03:22:15Z
dc.date.issued
2019-06
dc.identifier.citation
Fait, María Elisa; da Costa, Helen P. S.; Freitas, Cleverson D. T.; Bakas, Laura Susana; Morcelle del Valle, Susana Raquel; Antifungal activity of arginine-based surfactants; Bentham Science Publishers; Current Bioactive Compounds; 15; 3; 6-2019; 351-359
dc.identifier.issn
1573-4072
dc.identifier.uri
http://hdl.handle.net/11336/127886
dc.description.abstract
Amino acid based surfactants constitute an important class of surface active biomolecules showing remarkable biocompatible properties. Antimicrobial activity is one of the most remarkable biological properties of this kind of surfactants, which have been widely studied against a broad spectrum of microorganisms. However, the antifungal activity of this kind of compound has been less well investigated. The aim of this work is the study of the antifungal activity of two novel argininebased surfactants (Nα-benzoyl-arginine decylamide, Bz-Arg-NHC10 and Nα-benzoyl-arginine dodecylamide, Bz-Arg-NHC12), obtained by an enzymatic strategy, against phytopathogenic filamentous fungi and dermatophyte strains. Methods: Four phytopathogenic fungi (Fusarium oxysporum, Fusarium solani, Colletotrichum gloeosporioides and Colletotrichum lindemuthianum) and two human pathogenic fungi (dermatophytes Trichophyton rubrum and Trichophyton mentagrophytes) were tested. Inhibition of vegetative growth and conidia germination was investigated for the phytopathogenic fungi. In order to elucidate the possible mechanism of biocide action, membrane integrity, as well as the production of reactive oxygen species (ROS) were evaluated. Additionally, the inhibition of germination of dermatophyte microconidia due to both arginine-based surfactants was studied. Minimum inhibitory concentration, as well as the concentration that inhibits 50% of germination were determined for both compounds and both fungal strains. Results: For the vegetative growth of phytopathogenic fungi, the most potent arginine-based compound was Bz-Arg-NHC10. All the tested compounds interfered with the conidia development of the studied species. Investigation of the possible mechanism of toxicity towards phytopathogenic fungi indicated direct damage of the plasma membrane and production of ROS. For the two strains of dermatophyte fungi tested, all the proved compounds showed similar fungistatic efficacy. Conclusion: Bz-Arg-NHC10 and Bz-Arg-NHC12 were demonstrated to have broad biocidal ability against the proliferative vegetative form and the asexual reproductive conidia. Results suggest that both membrane permeabilization and induction of oxidative stress are part of the antifungal mechanisms involved in the interruption of normal conidia development by Bz-Arg-NHCn, leading to cell death.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Bentham Science Publishers
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ANTIFUNGAL ACTIVITY
dc.subject
ARGININE-BASED SURFACTANTS
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DERMATOPHYTES
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MEMBRANE DAMAGE
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PHYTOPATHOGENIC FUNGI
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ROS PRODUCTION
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Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Antifungal activity of arginine-based surfactants
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-03-05T18:32:15Z
dc.identifier.eissn
1875-6646
dc.journal.volume
15
dc.journal.number
3
dc.journal.pagination
351-359
dc.journal.pais
Emiratos Árabes Unidos
dc.journal.ciudad
Abu Dhabi
dc.description.fil
Fil: Fait, María Elisa. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
dc.description.fil
Fil: da Costa, Helen P. S.. Universidade Federal Do Ceara; Brasil
dc.description.fil
Fil: Freitas, Cleverson D. T.. Universidade Federal Do Ceara; Brasil
dc.description.fil
Fil: Bakas, Laura Susana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina
dc.description.fil
Fil: Morcelle del Valle, Susana Raquel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
dc.journal.title
Current Bioactive Compounds
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.eurekaselect.com/node/159535/article/antifungal-activity-of-arginine-based-surfactants
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.2174/1573407214666180131161302
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