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dc.contributor.author
Mejia, Rojelio  
dc.contributor.author
Damania, Ashish  
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Jeun, Rebecca  
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Bryan, Patricia E.  
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Vargas, Paola  
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Juarez, Marisa del Valle  
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Cajal, Silvana Pamela  
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Nasser, Julio Rubén  
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Krolewiecki, Alejandro Javier  
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Lefoulon, Emilie  
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Long, Courtney  
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Drake, Evan  
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Cimino, Rubén Oscar  
dc.contributor.author
Slatko, Barton  
dc.date.available
2021-03-10T02:49:54Z  
dc.date.issued
2020-04  
dc.identifier.citation
Mejia, Rojelio; Damania, Ashish; Jeun, Rebecca; Bryan, Patricia E.; Vargas, Paola; et al.; Impact of intestinal parasites on microbiota and cobalamin gene sequences: A pilot study; BioMed Central; Parasites and Vectors; 13; 1; 4-2020; 1-9  
dc.identifier.issn
1756-3305  
dc.identifier.uri
http://hdl.handle.net/11336/127879  
dc.description.abstract
Background: Approximately 30% of children worldwide are infected with gastrointestinal parasites. Depending on the species, parasites can disrupt intestinal bacterial microbiota affecting essential vitamin biosynthesis. Methods: Stool samples were collected from 37 asymptomatic children from a previous cross-sectional Argentinian study. A multi-parallel real-time quantitative PCR was implemented for Ascaris lumbricoides, Ancylostoma duodenale, Necator americanus, Strongyloides stercoralis, Trichuris trichiura, Cryptosporidium spp., Entamoeba histolytica and Giardia duodenalis. In addition, whole-genome sequencing analysis was conducted for bacterial microbiota on all samples and analyzed using Livermore Metagenomic Analysis Toolkit and DIAMOND software. Separate analyses were carried out for uninfected, Giardia-only, Giardia + helminth co-infections, and helminth-only groups. Results: For Giardia-only infected children compared to uninfected children, DNA sequencing data showed a decrease in microbiota biodiversity that correlated with increasing Giardia burden and was statistically significant using Shannon's alpha diversity (Giardia-only > 1 fg/μl 2.346; non-infected group 3.253, P = 0.0317). An increase in diversity was observed for helminth-only infections with a decrease in diversity for Giardia + helminth co-infections (P = 0.00178). In Giardia-only infections, microbiome taxonomy changed from Firmicutes towards increasing proportions of Prevotella, with the degree of change related to the intensity of infection compared to uninfected (P = 0.0317). The abundance of Prevotella bacteria was decreased in the helminths-only group but increased for Giardia + helminth co-infections (P = 0.0262). Metagenomic analysis determined cobalamin synthesis was decreased in the Giardia > 1 fg/μl group compared to both the Giardia < 1 fg/μl and the uninfected group (P = 0.0369). Giardia + helminth group also had a decrease in cobalamin CbiM genes from helminth-only infections (P = 0.000754). Conclusion: The study results may provide evidence for an effect of parasitic infections enabling the permissive growth of anaerobic bacteria such as Prevotella, suggesting an altered capacity of vitamin B12 (cobalamin) biosynthesis and potential impact on growth and development in children.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
BioMed Central  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/  
dc.subject
COBALAMIN  
dc.subject
GIARDIA DUODENALIS  
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HELMINTHS  
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MICROBIOME  
dc.subject.classification
Parasitología  
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Ciencias de la Salud  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Impact of intestinal parasites on microbiota and cobalamin gene sequences: A pilot study  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2021-03-05T18:33:18Z  
dc.journal.volume
13  
dc.journal.number
1  
dc.journal.pagination
1-9  
dc.journal.pais
Reino Unido  
dc.journal.ciudad
Londres  
dc.description.fil
Fil: Mejia, Rojelio. Baylor College of Medicine; Estados Unidos. Universidad Nacional de Salta; Argentina  
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Fil: Damania, Ashish. Baylor College of Medicine; Estados Unidos  
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Fil: Jeun, Rebecca. Baylor College of Medicine; Estados Unidos  
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Fil: Bryan, Patricia E.. Baylor College of Medicine; Estados Unidos  
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Fil: Vargas, Paola. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina  
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Fil: Juarez, Marisa del Valle. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina  
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Fil: Cajal, Silvana Pamela. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina  
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Fil: Nasser, Julio Rubén. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina  
dc.description.fil
Fil: Krolewiecki, Alejandro Javier. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina  
dc.description.fil
Fil: Lefoulon, Emilie. New England Biolabs; Estados Unidos  
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Fil: Long, Courtney. New England Biolabs; Estados Unidos  
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Fil: Drake, Evan. New England Biolabs; Estados Unidos  
dc.description.fil
Fil: Cimino, Rubén Oscar. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina  
dc.description.fil
Fil: Slatko, Barton. New England Biolabs; Estados Unidos  
dc.journal.title
Parasites and Vectors  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-04073-7  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/s13071-020-04073-7