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dc.contributor.author
Mejia, Rojelio
dc.contributor.author
Damania, Ashish
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Jeun, Rebecca
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Bryan, Patricia E.
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Vargas, Paola
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Juarez, Marisa del Valle
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Cajal, Silvana Pamela
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Nasser, Julio Rubén
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Krolewiecki, Alejandro Javier
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Lefoulon, Emilie
dc.contributor.author
Long, Courtney
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Drake, Evan
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Cimino, Rubén Oscar
dc.contributor.author
Slatko, Barton
dc.date.available
2021-03-10T02:49:54Z
dc.date.issued
2020-04
dc.identifier.citation
Mejia, Rojelio; Damania, Ashish; Jeun, Rebecca; Bryan, Patricia E.; Vargas, Paola; et al.; Impact of intestinal parasites on microbiota and cobalamin gene sequences: A pilot study; BioMed Central; Parasites and Vectors; 13; 1; 4-2020; 1-9
dc.identifier.issn
1756-3305
dc.identifier.uri
http://hdl.handle.net/11336/127879
dc.description.abstract
Background: Approximately 30% of children worldwide are infected with gastrointestinal parasites. Depending on the species, parasites can disrupt intestinal bacterial microbiota affecting essential vitamin biosynthesis. Methods: Stool samples were collected from 37 asymptomatic children from a previous cross-sectional Argentinian study. A multi-parallel real-time quantitative PCR was implemented for Ascaris lumbricoides, Ancylostoma duodenale, Necator americanus, Strongyloides stercoralis, Trichuris trichiura, Cryptosporidium spp., Entamoeba histolytica and Giardia duodenalis. In addition, whole-genome sequencing analysis was conducted for bacterial microbiota on all samples and analyzed using Livermore Metagenomic Analysis Toolkit and DIAMOND software. Separate analyses were carried out for uninfected, Giardia-only, Giardia + helminth co-infections, and helminth-only groups. Results: For Giardia-only infected children compared to uninfected children, DNA sequencing data showed a decrease in microbiota biodiversity that correlated with increasing Giardia burden and was statistically significant using Shannon's alpha diversity (Giardia-only > 1 fg/μl 2.346; non-infected group 3.253, P = 0.0317). An increase in diversity was observed for helminth-only infections with a decrease in diversity for Giardia + helminth co-infections (P = 0.00178). In Giardia-only infections, microbiome taxonomy changed from Firmicutes towards increasing proportions of Prevotella, with the degree of change related to the intensity of infection compared to uninfected (P = 0.0317). The abundance of Prevotella bacteria was decreased in the helminths-only group but increased for Giardia + helminth co-infections (P = 0.0262). Metagenomic analysis determined cobalamin synthesis was decreased in the Giardia > 1 fg/μl group compared to both the Giardia < 1 fg/μl and the uninfected group (P = 0.0369). Giardia + helminth group also had a decrease in cobalamin CbiM genes from helminth-only infections (P = 0.000754). Conclusion: The study results may provide evidence for an effect of parasitic infections enabling the permissive growth of anaerobic bacteria such as Prevotella, suggesting an altered capacity of vitamin B12 (cobalamin) biosynthesis and potential impact on growth and development in children.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
BioMed Central
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
COBALAMIN
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GIARDIA DUODENALIS
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HELMINTHS
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MICROBIOME
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Parasitología
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Ciencias de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Impact of intestinal parasites on microbiota and cobalamin gene sequences: A pilot study
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-03-05T18:33:18Z
dc.journal.volume
13
dc.journal.number
1
dc.journal.pagination
1-9
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Mejia, Rojelio. Baylor College of Medicine; Estados Unidos. Universidad Nacional de Salta; Argentina
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Fil: Damania, Ashish. Baylor College of Medicine; Estados Unidos
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Fil: Jeun, Rebecca. Baylor College of Medicine; Estados Unidos
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Fil: Bryan, Patricia E.. Baylor College of Medicine; Estados Unidos
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Fil: Vargas, Paola. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
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Fil: Juarez, Marisa del Valle. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
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Fil: Cajal, Silvana Pamela. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
dc.description.fil
Fil: Nasser, Julio Rubén. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
dc.description.fil
Fil: Krolewiecki, Alejandro Javier. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina
dc.description.fil
Fil: Lefoulon, Emilie. New England Biolabs; Estados Unidos
dc.description.fil
Fil: Long, Courtney. New England Biolabs; Estados Unidos
dc.description.fil
Fil: Drake, Evan. New England Biolabs; Estados Unidos
dc.description.fil
Fil: Cimino, Rubén Oscar. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina
dc.description.fil
Fil: Slatko, Barton. New England Biolabs; Estados Unidos
dc.journal.title
Parasites and Vectors
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-04073-7
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/s13071-020-04073-7
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