Repositorio Institucional
Repositorio Institucional
CONICET Digital
  • Inicio
  • EXPLORAR
    • AUTORES
    • DISCIPLINAS
    • COMUNIDADES
  • Estadísticas
  • Novedades
    • Noticias
    • Boletines
  • Ayuda
    • General
    • Datos de investigación
  • Acerca de
    • CONICET Digital
    • Equipo
    • Red Federal
  • Contacto
JavaScript is disabled for your browser. Some features of this site may not work without it.
  • INFORMACIÓN GENERAL
  • RESUMEN
  • ESTADISTICAS
 
Artículo

Carbonic anhydrase II/sodium-proton exchanger 1 metabolon complex in cardiomyopathy of ob−/− type 2 diabetic mice

Jaquenod de Giusti, CarolinaIcon ; Blanco, Paula GracielaIcon ; Lamas, Paula Ayelén; Carrizo Velasquez, Fernanda Elisabeth; Lofeudo, Juan ManuelIcon ; Portiansky, Enrique LeoIcon ; Alvarez, BernardoIcon
Fecha de publicación: 11/2019
Editorial: Academic Press Ltd - Elsevier Science Ltd
Revista: Journal of Molecular and Cellular Cardiology
ISSN: 0022-2828
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Fisiología

Resumen

Heart failure is the leading cause of death among diabetic people. Cellular and molecular entities leading to diabetic cardiomyopathy are, however, poorly understood. Coupling of cardiac carbonic anhydrase II (CAII) and Na+/H+ exchanger 1 (NHE1) to form a transport metabolon was analyzed in obese type 2 diabetic mice (ob−/−) and control heterozygous littermates (ob+/−). Echocardiography showed elevated systolic interventricular septum thickness and systolic posterior wall thickness in ob−/− mice at 9 and 16 weeks. ob−/− mice showed increased left ventricular (LV) weight/tibia length ratio and increased cardiomyocyte cross sectional area as compared to controls, indicating cardiac hypertrophy. Immunoblot analysis showed increased CAII expression in LV samples of ob−/− vs. ob+/− mice, and augmented Ser703 phosphorylation on NHE1 in ob−/− hearts. Reciprocal co-immunoprecipitation analysis showed strong association of CAII and NHE1 in LV samples of ob−/− mice. NHE1-dependent rate of intracellular pH (pHi) normalization after transient acid loading of isolated cardiomyocytes was higher in ob−/− mice vs. ob+/−. NHE transport activity was also augmented in cultured H9C2 rat cardiomyoblasts treated with high glucose/high palmitate, and it was normalized after CA inhibition. We conclude that the NHE1/CAII metabolon complex is exacerbated in diabetic cardiomyopathy of ob−/− mice, which may lead to perturbation of pHi and [Na+] and [Ca2+] handling in these diseased hearts.
Palabras clave: CARBONIC ANHYDRASE II , DIABETIC CARDIOMYOPATHY , INTRACELLULAR PH , NHE1
Ver el registro completo
 
Archivos asociados
Tamaño: 1.335Mb
Formato: PDF
.
Solicitar
Licencia
info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/127849
DOI: http://dx.doi.org/10.1016/j.yjmcc.2019.09.005
URL: https://www.sciencedirect.com/science/article/pii/S0022282818312550
Colecciones
Articulos(CCT - LA PLATA) [7034]
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Citación
Jaquenod de Giusti, Carolina; Blanco, Paula Graciela; Lamas, Paula Ayelén; Carrizo Velasquez, Fernanda Elisabeth; Lofeudo, Juan Manuel; et al.; Carbonic anhydrase II/sodium-proton exchanger 1 metabolon complex in cardiomyopathy of ob−/− type 2 diabetic mice; Academic Press Ltd - Elsevier Science Ltd; Journal of Molecular and Cellular Cardiology; 136; 11-2019; 53-63
Compartir
Altmétricas
 
Estadísticas
Visualizaciones: 65
Descargas: 0

Enviar por e-mail
Separar cada destinatario (hasta 5) con punto y coma.
  • Facebook
  • Twitter
  • Instagram
  • YouTube
  • Sound Cloud

Los contenidos del CONICET están licenciados bajo Creative Commons Reconocimiento 2.5 Argentina License

Ministerio
https://www.conicet.gov.ar/ - CONICET

Inicio

Explorar

  • Autores
  • Disciplinas
  • Comunidades

Estadísticas

Novedades

  • Noticias
  • Boletines

Ayuda

Acerca de

  • CONICET Digital
  • Equipo
  • Red Federal

Contacto

Godoy Cruz 2290 (C1425FQB) CABA – República Argentina – Tel: +5411 4899-5400 repositorio@conicet.gov.ar
TÉRMINOS Y CONDICIONES