In vitro experiments and infrared spectroscopy analysis of acid and alkaline phosphatase inhibition by vanadium complexes

Abstract

In the course of our research of vanadium-containing complexes, two oxidovanadium complexes containing vanadium(V) and (IV) core with 4-aminobenzoic acid and/or peroxo anion as ligands were synthesized and characterized by elemental analysis, conductivity measurements, thermogravimetric analysis, and spectroscopies.(([VO(O 2 )(C 7 H 6 NO 2 )(H 2 O)] . H 2 O, [VO(C 7 H 6 NO 2 ) 2 H 2 O]) were supported by experimental data and theoretical studies (Density functional theory, DFT). The complexes were evaluated in vitro as phosphatase inhibitors (alkaline and acid enzymes) being considered as potential pharmaceutical agents under over-expression of those biochemical markers.The effect achieved was then analysed through FTIR spectroscopy. Changes in the finger print substrate bands as well as induced conformational changes on phosphatases enzymes secondary structure were further examined. Eventually albumin interactions experimentswere performed in order to derive their bioavailability.