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dc.contributor.author
Dai, Shunhong
dc.contributor.author
Petruccelli, Silvana
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dc.contributor.author
Ordiz, Maria Isabel
dc.contributor.author
Zhang, Zhihong
dc.contributor.author
Chen, Shouyi
dc.contributor.author
Beachy, Roger N.
dc.date.available
2021-03-09T12:30:43Z
dc.date.issued
2003-09
dc.identifier.citation
Dai, Shunhong; Petruccelli, Silvana; Ordiz, Maria Isabel; Zhang, Zhihong; Chen, Shouyi; et al.; Functional analysis of RF2a, a rice transcription factor; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 278; 38; 9-2003; 36396-36402
dc.identifier.issn
0021-9258
dc.identifier.uri
http://hdl.handle.net/11336/127799
dc.description.abstract
RF2a is a bZIP transcription factor that regulates expression of the promoter of rice tungro bacilliform bad-navirus. RF2a is predicted to include three domains that contribute to its function. The results of transient assays with mutants of RF2a from which one or more domains were removed demonstrated that the acidic domain was essential for the activation of gene expression, although the proline-rich and glutamine-rich domains each played a role in this function. Studies using fusion proteins of different functional domains of RF2a with the 2C7 synthetic zinc finger DNA-binding domain showed that the acidic region is a relatively strong activation domain, the function of which is dependent on the context in which the domain is placed. Data from transgenic plants further supported the conclusion that the acidic domain was important for maintaining the biological function of RF2a. RF2a and TBP (TATA-binding protein) synergistically activate transcription in vitro (Zhu, Q., Ordiz, M. I., Dabi, T., Beachy, R. N., and Lamb, C. (2002) Plant Cell 14, 795-803). In vitro and in vivo assays showed that RF2a interacts with TBP through the glutamine-rich domain but not the acidic domain. Functional analysis of such interactions indicates that the acidic domain activates transcription through mechanisms other than via the direct recruitment of TBP.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Biochemistry and Molecular Biology
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Genetic engineering
dc.subject
Transcription
dc.subject
plant disease
dc.subject
rice tungro bacilliform virus
dc.subject.classification
Otras Ciencias Químicas
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dc.subject.classification
Ciencias Químicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Functional analysis of RF2a, a rice transcription factor
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-02-17T20:11:24Z
dc.journal.volume
278
dc.journal.number
38
dc.journal.pagination
36396-36402
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Bethesda, Maryland
dc.description.fil
Fil: Dai, Shunhong. Chinese Academy of Sciences; República de China. Donald Danforth Plant Science Center; Estados Unidos
dc.description.fil
Fil: Petruccelli, Silvana. Donald Danforth Plant Science Center; Estados Unidos. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
dc.description.fil
Fil: Ordiz, Maria Isabel. Donald Danforth Plant Science Center; Estados Unidos
dc.description.fil
Fil: Zhang, Zhihong. Donald Danforth Plant Science Center; Estados Unidos
dc.description.fil
Fil: Chen, Shouyi. Chinese Academy of Sciences; República de China
dc.description.fil
Fil: Beachy, Roger N.. Donald Danforth Plant Science Center; Estados Unidos
dc.journal.title
Journal of Biological Chemistry (online)
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1074/jbc.M304862200
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://tinyurl.com/yagg2yhm
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