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dc.contributor.author
Butassi, Estefanía  
dc.contributor.author
Svetaz, Laura Andrea  
dc.contributor.author
Zhou, Shuaizhen  
dc.contributor.author
Wolfender, Jean Luc  
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García Cortés, Juan Carlos  
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Ribas, Juan  
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Díaz, Caridad  
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Palacio, José Pérez del  
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Vicente Pérez, Francisca  
dc.contributor.author
Zacchino, Susana Alicia Stella  
dc.date.available
2021-03-05T19:51:36Z  
dc.date.issued
2019-07  
dc.identifier.citation
Butassi, Estefanía; Svetaz, Laura Andrea; Zhou, Shuaizhen; Wolfender, Jean Luc; García Cortés, Juan Carlos; et al.; The antifungal activity and mechanisms of action of quantified extracts from berries, leaves and roots of Phytolacca tetramera; Elsevier Gmbh; Phytomedicine; 60; 7-2019; 1-9; 152884  
dc.identifier.issn
0944-7113  
dc.identifier.uri
http://hdl.handle.net/11336/127681  
dc.description.abstract
Background: Phytolacca tetramera is an endemic plant from Argentina that is currently at serious risk because its environment is subjected to a high anthropic impact. A previous study has shown that berry extracts obtained from this plant display antifungal activity against multiple human-pathogenic fungi when tested with a non-standardized method. Further evidences of the antifungal properties of other parts of the plant and studies of mechanism of antifungal action of the antifungal chemically characterized extracts are required. Purpose: This study aimed to gain further evidence of the antifungal activity of P. tetramera berry, leaf and root extracts in order to find the most active extract to be developed as an Herbal Medicinal Antifungal Product. The medicinal usefulness of P. tetramera extracts as antifungal agents will serve as an important support to create concience and carry out actions tending to the preservation of this threatened species and its environment. Materials and methods: Chemical analysis of all P. tetramera extracts, including quantitation of selected markers, was performed through UHPLC-ESI-MS/MS and UPLC-ESI-MS techniques according to the European Medicines Agency (EMA). The antifungal activity of the quantified extracts was tested with the standardized CLSI microbroth dilution method against Candida spp. Antifungal mechanisms of the most active extract were studied by examination of morphological changes by phase-contrast and fluorescence microscopies and both, cellular and enzymatic assays targeting either the fungal membrane or the cell wall. Results: The antifungal activity of twelve P. tetramera extracts was tested against Candida albicans and Candida glabrata. The dichloromethane extract from berries (PtDEb) showed the best activity. Phytolaccagenin (PhytG) and phytolaccoside B (PhytB) were selected as the main active markers for the antifungal P. tetramera extracts. The quantitation of these active markers in all extracts showed that PtDEb possessed the highest amount of PhytG and PhytB. Finally, studies on the mechanism of antifungal action showed that the most active PtDEb extract produces morphological changes compatible with a damage of the cell wall and/or the plasma membrane. Cellular and enzymatic assays showed that PtDEb would not damage the fungal cell wall by itself, but would alter the plasma membrane. In agreement, PtDEb was found to bind to ergosterol, the main sterol of the fungal plasma membrane. Conclusion: Studies of the anti-Candida activity of P. tetramera extracts led to the selection of PtDEb as the most suitable extract, confirming the antifungal properties of the threatened species P. tetramera. The new data give a valuable reason for the definitive protection of this sp. and its natural environment thus allowing further studies for the future development of an Herbal Medicinal Antifungal Product.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Gmbh  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
CANDIDA ALBICANS  
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CANDIDA GLABRATA  
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PHYTOLACCA TETRAMERA  
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PHYTOLACCAGENIN  
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PHYTOLACCOSIDE B  
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QUANTIFIED EXTRACTS  
dc.subject.classification
Química Orgánica  
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Ciencias Químicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
The antifungal activity and mechanisms of action of quantified extracts from berries, leaves and roots of Phytolacca tetramera  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2020-11-18T20:50:36Z  
dc.journal.volume
60  
dc.journal.pagination
1-9; 152884  
dc.journal.pais
Alemania  
dc.journal.ciudad
Berlín  
dc.description.fil
Fil: Butassi, Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Orgánica. Área Farmacognosia; Argentina  
dc.description.fil
Fil: Svetaz, Laura Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina  
dc.description.fil
Fil: Zhou, Shuaizhen. Chinese Academy of Sciences; República de China. Universite de Lausanne; Suiza  
dc.description.fil
Fil: Wolfender, Jean Luc. Universite de Lausanne; Suiza  
dc.description.fil
Fil: García Cortés, Juan Carlos. Consejo Superior de Investigaciones Científicas; España. Universidad de Salamanca; España  
dc.description.fil
Fil: Ribas, Juan. Consejo Superior de Investigaciones Científicas; España. Universidad de Salamanca; España  
dc.description.fil
Fil: Díaz, Caridad. Fundación Medina; España. Parque Tecnológico de Ciencias de la Salud; España  
dc.description.fil
Fil: Palacio, José Pérez del. Fundación Medina; España. Parque Tecnológico de Ciencias de la Salud; España  
dc.description.fil
Fil: Vicente Pérez, Francisca. Fundación Medina; España. Parque Tecnológico de Ciencias de la Salud; España  
dc.description.fil
Fil: Zacchino, Susana Alicia Stella. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Orgánica. Área Farmacognosia; Argentina  
dc.journal.title
Phytomedicine  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.phymed.2019.152884  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0944711319300546