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dc.contributor.author
Lorenzo, María Soledad  
dc.contributor.author
Maruri, Alejandro  
dc.contributor.author
Cruzans, Paula Romina  
dc.contributor.author
Teplitz, Gabriela Maia  
dc.contributor.author
Tello, M.F.  
dc.contributor.author
Lombardo, Daniel Marcelo  
dc.date.available
2021-03-05T19:27:20Z  
dc.date.issued
2019-06-27  
dc.identifier.citation
Lorenzo, María Soledad; Maruri, Alejandro; Cruzans, Paula Romina; Teplitz, Gabriela Maia; Tello, M.F.; et al.; The antioxidant dimethylthiourea improves IVF efficiency and decreases cumulus cell apoptosis in pigs; Csiro Publishing; Reproduction Fertility and Development; 31; 10; 27-6-2019; 1607-1615  
dc.identifier.issn
1031-3613  
dc.identifier.uri
http://hdl.handle.net/11336/127671  
dc.description.abstract
Abattoir ovaries, which are the main source of oocytes for reproductive biotechnologies, arrive at the laboratory under ischaemic conditions. Reoxygenation generates reactive oxygen species (ROS) in ischaemic tissues, which could affect oocyte quality. The aim of this study was to evaluate the effect of supplementation of media with dimethylthiourea (DMTU) during the collection and washing of cumulus-oocyte complexes (COC) on ROS levels, COC apoptosis and oocyte nuclear and cytoplasmic maturation. Thus, the collection (TCM-199) and washing (TCM-199 with 10% porcine follicular fluid, sodium pyruvate and antibiotics) media were supplemented with 1 and 10 mM DMTU. In the control group, the media were not supplemented with DMTU. Intracellular ROS levels decreased significantly in the DMTU-treated groups (P < 0.05). Although no effects on rate of nuclear maturation were observed, DMTU significantly increased sperm penetration rates without increasing polyspermy (P < 0.05). The addition of 10 mM DMTU to the collection and washing media enhanced IVF efficiency. DMTU did not modify the early or late apoptosis of oocytes. Both concentrations of DMTU significantly increased viability and decreased the apoptosis of cumulus cells (P < 0.05). These results suggest that the addition of 1 or 10 mM of DMTU to the media during the collection and washing of porcine COCs is useful for decreasing cumulus apoptosis mediated by ROS and for optimising the IVF of porcine oocytes.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Csiro Publishing  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
OOCYTE APOPTOSIS  
dc.subject
PORCINE OOCYTE MATURATION  
dc.subject
REACTIVE OXYGEN SPECIES (ROS) LEVELS  
dc.subject.classification
Otras Ciencias Veterinarias  
dc.subject.classification
Ciencias Veterinarias  
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CIENCIAS AGRÍCOLAS  
dc.title
The antioxidant dimethylthiourea improves IVF efficiency and decreases cumulus cell apoptosis in pigs  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2021-01-27T19:12:42Z  
dc.identifier.eissn
1448-5990  
dc.journal.volume
31  
dc.journal.number
10  
dc.journal.pagination
1607-1615  
dc.journal.pais
Australia  
dc.journal.ciudad
Collingwood  
dc.description.fil
Fil: Lorenzo, María Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriologías; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Maruri, Alejandro. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriologías; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Cruzans, Paula Romina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriologías; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Teplitz, Gabriela Maia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriologías; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Tello, M.F.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriologías; Argentina  
dc.description.fil
Fil: Lombardo, Daniel Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriologías; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.journal.title
Reproduction Fertility and Development  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.publish.csiro.au/rd/RD19020  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1071/RD19020