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dc.contributor.author
Hernández Alcántara, Annel M.
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Pardo, Sandra
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Mohedano, Mari Luz
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Vignolo, Graciela Margarita
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dc.contributor.author
de Moreno, Maria Alejandra
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Leblanc, Jean Guy Joseph
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Aznar, Rosa
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López, Paloma
dc.date.available
2021-03-02T20:48:24Z
dc.date.issued
2020-10
dc.identifier.citation
Hernández Alcántara, Annel M.; Pardo, Sandra; Mohedano, Mari Luz; Vignolo, Graciela Margarita; de Moreno, Maria Alejandra; et al.; The ability of riboflavin-overproducing lactiplantibacillus plantarum strains to survive under gastrointestinal conditions; Frontiers Media S.A.; Frontiers in Microbiology; 11; 10-2020; 1-17; 591945
dc.identifier.issn
1664-302X
dc.identifier.uri
http://hdl.handle.net/11336/127205
dc.description.abstract
Riboflavin, vitamin B2, is essential for humans and has to be obtained from the diet. Some lactic acid bacteria (LAB) produce this vitamin, and they can be used for in-situ fortification of foods. This could be an alternative to supplementation with chemically synthesized vitamin, to palliate riboflavin deficiencies in specific groups of people. Moreover, if the producing LAB could survive in the gastrointestinal stress (GIT) they could be added as probiotics in this environment. In the present study we tested two riboflavin-overproducing Lactiplantibacillus plantarum strains (M5MA1-B2 and M9MG6-B2), spontaneous mutants of LAB isolated from chicha, a traditional Andean beverage. These two LAB, and also their isogenic strains M5MA1-B2[pRCR12] and M9MG6-B2[pRCR12], expressing the mCherry protein from the pRCR12 plasmid, were evaluated in vitro under simulated GIT conditions. Among other, specifically developed protein fluorescence assays were used. The four LAB showed similar levels of adhesion (>6.0%) to Caco-2 cells, higher than that of the probiotic Lacticaseibacillus rhamnosus GG strain (4.51%). Thus, LAB biofilm formation was assessed in the labeled cells by intracellular mCherry fluorescence and in the unlabeled parental strains by crystal violet staining. Both methods detected the formation of consistent biofilms by the L. plantarum strains. The quantification of mCherry fluorescence was also used to analyze LAB auto-aggregation properties. High levels of auto-aggregation were detected for both M5MA1-B2[pRCR12] and M9MG6-B2[pRCR12]. Survival of LAB included in a commercial cereal-based food matrix (Incaparina) under GIT conditions was also evaluated. The four LAB were resistant in vitro to the stomach and intestinal stresses, and proliferated in this environment, indicating a protective and nutritional effect of the Incaparina on the bacteria. Also, M9MG6-B2 survival in the presence or absence of Incaparina was evaluated in vivo in a BALB/c mouse model. The administration of the M9MG6-B2 strain alone or together with Incaparina had no adverse effect on the health, growth and/or well-being of the rodents. In addition, an increment in the villus length/crypt depth ratio was observed. The overall results obtained indicate that the LAB studied have probiotic characteristics of interest for the development of functional foods.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Media S.A.
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
LACTIC ACID BACTERIA (LAB)
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LACTIPLANTIBACILLUS PLANTARUM
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PROTEIN MCHERRY
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RIBOFLAVIN
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TOLERANCE TO GASTROINTESTINAL STRESS OF LACTIPLANTIBACILLUS
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Otras Ingenierías y Tecnologías
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dc.subject.classification
Otras Ingenierías y Tecnologías
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dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
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dc.title
The ability of riboflavin-overproducing lactiplantibacillus plantarum strains to survive under gastrointestinal conditions
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-12-04T18:36:23Z
dc.journal.volume
11
dc.journal.pagination
1-17; 591945
dc.journal.pais
Suiza
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dc.journal.ciudad
Lausana
dc.description.fil
Fil: Hernández Alcántara, Annel M.. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Pardo, Sandra. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Mohedano, Mari Luz. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Vignolo, Graciela Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: Aznar, Rosa. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; España. Universidad de Valencia; España
dc.description.fil
Fil: López, Paloma. Consejo Superior de Investigaciones Científicas; España
dc.journal.title
Frontiers in Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fmicb.2020.591945
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2020.591945
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