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dc.contributor.author
Albarracín, Leonardo Miguel
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dc.contributor.author
Garcia-Castillo, Valeria
dc.contributor.author
Masumizu, Yuki
dc.contributor.author
Indo, Yuhki
dc.contributor.author
Islam, Md Aminul
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dc.contributor.author
Suda, Yoshihito
dc.contributor.author
García Cancino, Apolinaria
dc.contributor.author
Aso, Hisashi
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dc.contributor.author
Takahashi, Hideki
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dc.contributor.author
Kitazawa, Haruki
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dc.contributor.author
Villena, Julio Cesar
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dc.date.available
2021-03-02T20:35:51Z
dc.date.issued
2020-04-08
dc.identifier.citation
Albarracín, Leonardo Miguel; Garcia-Castillo, Valeria; Masumizu, Yuki; Indo, Yuhki; Islam, Md Aminul; et al.; Efficient Selection of New Immunobiotic Strains With Antiviral Effects in Local and Distal Mucosal Sites by Using Porcine Intestinal Epitheliocytes; Frontiers Media S.A.; Frontiers in Immunology; 11; 8-4-2020; 1-16; 543
dc.identifier.issn
1664-3224
dc.identifier.uri
http://hdl.handle.net/11336/127198
dc.description.abstract
Previously, we evaluated the effect of the immunobiotic strain Lactobacillus rhamnosus CRL1505 on the transcriptomic response of porcine intestinal epithelial (PIE) cells triggered by the challenge with the Toll-like receptor 3 (TLR-3) agonist poly(I:C) and successfully identified a group of genes that can be used as prospective biomarkers for the screening of new antiviral immunobiotics. In this work, several strains of lactobacilli were evaluated according to their ability to modulate the expression of IFNα, IFNβ, RIG1, TLR3, OAS1, RNASEL, MX2, A20, CXCL5, CCL4, IL-15, SELL, SELE, EPCAM, PTGS2, PTEGES, and PTGER4 in PIE cells after the stimulation with poly(I:C). Comparative analysis of transcripts variations revealed that one of the studied bacteria, Lactobacillus plantarum MPL16, clustered together with the CRL1505 strain, indicating a similar immunomodulatory potential. Two sets of in vivo experiments in Balb/c mice were performed to evaluate L. plantarum MPL16 immunomodulatory activities. Orally administered MPL16 prior intraperitoneal injection of poly(I:C) significantly reduced the levels of the proinflammatory mediators tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), and IL-15 in the intestinal mucosa. In addition, orally administered L. plantarum MPL16 prior nasal stimulation with poly(I:C) or respiratory syncytial virus infection significantly decreased the levels of the biochemical markers of lung tissue damage. In addition, reduced levels of the proinflammatory mediators TNF-α, IL-6, and IL-8 were found in MPL16-treated mice. Improved levels of IFN-β and IFN-γ in the respiratory mucosa were observed in mice treated with L. plantarum MPL16 when compared to control mice. The immunological changes induced by L. plantarum MPL16 were not different from those previously reported for the CRL1505 strain in in vitro and in vivo studies. The results of this work confirm that new immunobiotic strains with the ability of stimulating both local and distal antiviral immune responses can be efficiently selected by evaluating the expression of biomarkers in PIE cells.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Media S.A.
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
ANTIVIRAL RESPONSE
dc.subject
LACTOBACILLUS PLANTARUM MPL16
dc.subject
LACTOBACILLUS RHAMNOSUS CRL1505
dc.subject
PORCINE INTESTINAL EPITHELIAL CELLS
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RESPIRATORY IMMUNITY
dc.subject
TLR3
dc.subject.classification
Inmunología
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Efficient Selection of New Immunobiotic Strains With Antiviral Effects in Local and Distal Mucosal Sites by Using Porcine Intestinal Epitheliocytes
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-10-06T19:12:05Z
dc.identifier.eissn
1664-3224
dc.journal.volume
11
dc.journal.pagination
1-16; 543
dc.journal.pais
Suiza
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dc.journal.ciudad
Lausanne
dc.description.fil
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
dc.description.fil
Fil: Garcia-Castillo, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad de Concepción; Chile
dc.description.fil
Fil: Masumizu, Yuki. Tohoku University; Japón
dc.description.fil
Fil: Indo, Yuhki. Tohoku University; Japón
dc.description.fil
Fil: Islam, Md Aminul. Tohoku University; Japón
dc.description.fil
Fil: Suda, Yoshihito. Miyagi University; Japón
dc.description.fil
Fil: García Cancino, Apolinaria. Universidad de Concepción; Chile
dc.description.fil
Fil: Aso, Hisashi. Tohoku University; Japón
dc.description.fil
Fil: Takahashi, Hideki. Tohoku University; Japón
dc.description.fil
Fil: Kitazawa, Haruki. Tohoku University; Japón
dc.description.fil
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
dc.journal.title
Frontiers in Immunology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fimmu.2020.00543
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2020.00543
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