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dc.contributor.author
Gervasi, Maria Gracia
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dc.contributor.author
Marczylo, Timothy H.
dc.contributor.author
Lam, Patricia
dc.contributor.author
Rama, Shashi
dc.contributor.author
Franchi, Ana Maria
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dc.contributor.author
Konje, Justin C.
dc.contributor.author
Perez Martinez, Silvina Laura
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dc.date.available
2015-07-17T13:31:06Z
dc.date.issued
2013-08
dc.identifier.citation
Gervasi, Maria Gracia; Marczylo, Timothy H.; Lam, Patricia; Rama, Shashi; Franchi, Ana Maria; et al.; Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle; Public Library Science; Plos One; 8; 8; 8-2013; e72521
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/1256
dc.description.abstract
Mammalian oviduct acts as a reservoir for spermatozoa and provides an environment in which they may compete for the opportunity to fertilize the oocyte. Whilst in the oviduct spermatozoa undergo capacitation essential for fertilization. Sperm-oviduct interaction is essential for sperm capacitation and is a tightly regulated process influenced by the local microenvironment. Previously we reported that the endocannabinoid anandamide (AEA) regulates sperm release from epithelial oviductal cells by promoting sperm capacitation. The aims of this work were to measure the AEA content and to characterize the main AEA metabolic pathway in the bovine oviduct and determine how these change through the oestrous cycle. In this study, the levels of AEA and two other -N-acylethanolamines, N-oleoylethanolamine and N-palmitoylethanolamine, were measured in bovine oviduct collected during different stages of oestrous cycle by ultra high performance liquid chromatography tandem mass spectrometry. Results indicated that intracellular oviductal epithelial levels of all three N-acylethanolamines fluctuate during oestrous cycle. Anandamide from oviductal fluid also varied during oestrous cycle, with the highest values detected during the periovulatory period. Endocannabinoid levels from ipsilateral oviduct to ovulation were higher than those detected in the contralateral one, suggesting that levels of oviductal AEA may be regulated by ovarian hormones. The expression and localization of N-acylethanolamines metabolizing enzymes in bovine oviduct were also determined by RT-PCR, Western blot, and immunohistochemistry but no change was found during the oestrous cycle. Furthermore, nanomolar levels of AEA were detected in follicular fluids, suggesting that during ovulation the mature follicle may contribute to oviductal AEA levels to create an endocannabinoid gradient conducive to the regulation of sperm function for successful fertilization.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Anandamide
dc.subject
Oviductal Fluids
dc.subject
Estrous Cycle
dc.subject
Bovine
dc.subject.classification
Biología Reproductiva
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
8
dc.journal.number
8
dc.journal.pagination
e72521
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Gervasi, Maria Gracia. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina;
dc.description.fil
Fil: Marczylo, Timothy H.. University of Leicester; Reino Unido;
dc.description.fil
Fil: Lam, Patricia. University of Leicester; Reino Unido;
dc.description.fil
Fil: Rama, Shashi. University of Leicester; Reino Unido;
dc.description.fil
Fil: Franchi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina;
dc.description.fil
Fil: Konje, Justin C.. University of Leicester; Reino Unido;
dc.description.fil
Fil: Perez Martinez, Silvina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina;
dc.journal.title
Plos One
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0072521
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