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dc.contributor.author
Juri Ayub, Maximiliano
dc.contributor.author
Ma, Kit-Wan
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Shaw, Pang-Chui
dc.contributor.author
Wong, Kam-Bo
dc.date.available
2021-02-08T12:36:59Z
dc.date.issued
2008-03
dc.identifier.citation
Juri Ayub, Maximiliano; Ma, Kit-Wan; Shaw, Pang-Chui; Wong, Kam-Bo; Trypanosoma cruzi: High ribosomal resistance to trichosanthin inactivation; Academic Press Inc Elsevier Science; Experimental Parasitology; 118; 3; 3-2008; 442-447
dc.identifier.issn
0014-4894
dc.identifier.uri
http://hdl.handle.net/11336/125061
dc.description.abstract
Trypanosoma cruzi is the parasite causing Chagas Disease. Several results already published suggest that T. cruzi ribosomes have remarkable differences with their mammalian counterparts. In the present work, we showed that trypanosomatid (T. cruzi and Crithidia fasciculata) ribosomes are highly resistant to inactivation by trichosanthin (TCS), which is active against mammalian ribosomes. Differential resistance is an intrinsic feature of the ribosomal particles, as demonstrated by using assays where the only variable was the ribosomes source. Because we have recently described that TCS interacts with the acidic C-terminal end of mammalian ribosomal P proteins, we assayed the effect of a TCS variant, which is unable to interact with P proteins, on trypanosomatid ribosomes. This mutant showed similar shifting of IC50 values on rat, T. cruzi and C. fasciculata ribosomes, suggesting that the resistance mechanism might involve other ribosomal components rather than the C-terminal end of P proteins.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Press Inc Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CHAGAS DISEASE
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PROTEIN SYNTHESIS
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RIBOSOME-INACTIVATING PROTEIN (RIP)
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TRICHOSANTHIN
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TRYPANOSOMA
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Trypanosoma cruzi: High ribosomal resistance to trichosanthin inactivation
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-07-22T15:44:05Z
dc.journal.volume
118
dc.journal.number
3
dc.journal.pagination
442-447
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Juri Ayub, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires; Argentina
dc.description.fil
Fil: Ma, Kit-Wan. The Chinese University of Hong Kong; China
dc.description.fil
Fil: Shaw, Pang-Chui. The Chinese University of Hong Kong; China
dc.description.fil
Fil: Wong, Kam-Bo. The Chinese University of Hong Kong; China
dc.journal.title
Experimental Parasitology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0014489407002408
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.exppara.2007.09.002
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