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dc.contributor.author
Mercadal, Pablo Agustin
dc.contributor.author
Encina, Ezequiel Roberto
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Coronado, Eduardo A.
dc.date.available
2021-01-29T14:26:02Z
dc.date.issued
2019-09
dc.identifier.citation
Mercadal, Pablo Agustin; Encina, Ezequiel Roberto; Coronado, Eduardo A.; Colloidal SERS Substrate for the Ultrasensitive Detection of Biotinylated Antibodies Based on Near-Field Gradient within the Gap of Au Nanoparticle Dimers; American Chemical Society; Journal of Physical Chemistry C; 123; 38; 9-2019; 23577-23585
dc.identifier.issn
1932-7447
dc.identifier.uri
http://hdl.handle.net/11336/124190
dc.description.abstract
Surface-enhanced Raman spectroscopy (SERS) has demonstrated to be a powerful technique for the ultrasensitive detection of different types of analytes and particularly biomolecules, with the rational design of SERS substrates being one of the most relevant issues for the development of effective detection protocols. In this work, a colloidal SERS substrate consisting of a pair of Au nanorods linked by the molecular bridge biotin/streptavidin/biotin has been obtained and employed for the detection of picomolar quantities of the biotinylated antibodies gliadin IgG and Ara h1 IgG, which is of great interest in food science. As a consequence of the bioconjugation strategy implemented, the SERS substrate, that is, the Au nanorod dimer, presents the advantage of combining in a single nanostructure the capabilities for both direct and indirect detection of the biotinylated antibodies. Furthermore, the experimental results are supported by detailed electrodynamics simulations which takes into account not only the gradient of the near-field enhancements within the hot spot but also the volume occupied by the respective biomolecules. The SERS substrate presented here could be straightforwardly employed for the ultrasensitive detection of other biotinylated biomolecules.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Chemical Society
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
DIMERS
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GOLD NANOPARTICLES
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ANTIBODY
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DETECTION
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Química Coloidal
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Colloidal SERS Substrate for the Ultrasensitive Detection of Biotinylated Antibodies Based on Near-Field Gradient within the Gap of Au Nanoparticle Dimers
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-11-17T18:34:01Z
dc.journal.volume
123
dc.journal.number
38
dc.journal.pagination
23577-23585
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Mercadal, Pablo Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.description.fil
Fil: Encina, Ezequiel Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.description.fil
Fil: Coronado, Eduardo A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.journal.title
Journal of Physical Chemistry C
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/acs.jpcc.9b02974
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1021/acs.jpcc.9b02974
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