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dc.contributor.author
Escobar Correas, Sophia Melanie
dc.contributor.author
Mendoza Porras, Omar
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Dellagnola, Federico A.
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Colgrave, Michelle L.
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Vega, Israel Aníbal
dc.date.available
2021-01-28T02:50:06Z
dc.date.issued
2019-07-19
dc.identifier.citation
Escobar Correas, Sophia Melanie; Mendoza Porras, Omar; Dellagnola, Federico A.; Colgrave, Michelle L.; Vega, Israel Aníbal; Integrative Proteomic Analysis of Digestive Tract Glycosidases from the Invasive Golden Apple Snail, Pomacea canaliculata; American Chemical Society; Journal of Proteome Research; 18; 9; 19-7-2019; 3342-3352
dc.identifier.issn
1535-3893
dc.identifier.uri
http://hdl.handle.net/11336/123988
dc.description.abstract
The freshwater snail Pomacea canaliculata, an invasive species of global significance, possesses a well-developed digestive system and diverse feeding mechanisms enabling the intake of a wide variety of food. The identification of glycosidases in adult snails would increase the understanding of their digestive physiology and potentially generate new opportunities to eradicate and/or control this invasive species. In this study, liquid chromatography coupled to tandem mass spectrometry was applied to define the occurrence, diversity, and origin of glycoside hydrolases along the digestive tract of P. canaliculata. A range of cellulases, hemicellulases, amylases, maltases, fucosidases, and galactosidases were identified across the digestive tract. The digestive gland and the contents of the crop and style sac yield a higher diversity of glycosidase-derived peptides. Subsequently, peptides derived from 81 glycosidases (46 proteins from the public database and 35 uniquely from the transcriptome database) that were distributed among 13 glycoside hydrolase families were selected and quantified using multiple reaction monitoring mass spectrometry. This study showed a high glycosidase abundance and diversity in the gut contents of P. canaliculata which participate in extracellular digestion of complex dietary carbohydrates. Salivary and digestive glands were the main tissues involved in their synthesis and secretion.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Chemical Society
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
AMPULLARIIDS
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AMYLASES
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CELLULASES
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DIGESTION
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FUCOSIDASES
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GALACTOSIDASES
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HEMICELLULASES
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LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY (LC-MS)
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MALTASES
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Zoología, Ornitología, Entomología, Etología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Integrative Proteomic Analysis of Digestive Tract Glycosidases from the Invasive Golden Apple Snail, Pomacea canaliculata
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-11-19T21:58:46Z
dc.journal.volume
18
dc.journal.number
9
dc.journal.pagination
3342-3352
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Escobar Correas, Sophia Melanie. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Fisiología; Argentina
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Fil: Mendoza Porras, Omar. Commonwealth Scientific And Industrial Research Organization; Australia
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Fil: Dellagnola, Federico A.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Fisiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
dc.description.fil
Fil: Colgrave, Michelle L.. Commonwealth Scientific And Industrial Research Organization; Australia
dc.description.fil
Fil: Vega, Israel Aníbal. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Universidad Nacional de Cuyo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
dc.journal.title
Journal of Proteome Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1021/acs.jproteome.9b00282
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acs.jproteome.9b00282
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