Artículo
Formulation and quality control of semi-solid containing harmless bacteria by-products: chronic wounds pro-healing activity
Sesto Cabral, María Eugenia
; Ramos Vernieri, Alberto Nicolás
; Macedo, Alexandre Jose ; Trentin, Danielle; Treter, Yaninne; Manzo, Ruben Hilario
; Valdez, Juan Carlos
Fecha de publicación:
2015
Editorial:
Taylor & Francis
Revista:
Pharmaceutical Development And Technology
ISSN:
1083-7450
e-ISSN:
1097-9867
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Chronic wounds are those that remain in a chronic inflammatory state and fail to follow normal healing process. Infection is one of the most important causes of chronicity. A frequent pathogen isolated from chronic infections is Pseudomonas aeruginosa; refractory to therapy and host immune attack in its biofilm phenotype. Lactobacillus plantarum cultures supernatants (LAPS) interfere with its pathogenic capacity. In addition, LAPS showed bacteriostatic and bactericide properties and is neither cytotoxic nor an inductor of necrosis-apoptosis. LAPSs chemical composition was determined; allowing us to propose a correlation between its constituents and their biological activity. This article shows a pharmaceutical dosage form designed by using LAPS as an API with pro-healing activity and its quality control. Pharmacotechnical and anti-microbial assays were adapted to demonstrate that the vehicle used does not modify LAPS activities. Selected formulation (F100) showed fair mechanical and technological properties. From the in vitro release assays was found an adequate release from the carrier matrix and maintains its anti-pathogenic activity for 6 months. We propose F100 for chronic wounds treatment. The use of harmless bacteria by-products, such as LAPS, to antagonize infectious pathogens that have ability to form biofilm is an efficient and economic approach to treat infected chronic wounds.
Palabras clave:
Chronic Wounds
,
Bacteria by Products
,
Formulation
,
Quality Control
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - NOA SUR)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NOA SUR
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NOA SUR
Citación
Sesto Cabral, María Eugenia; Ramos Vernieri, Alberto Nicolás; Macedo, Alexandre Jose ; Trentin, Danielle; Treter, Yaninne; et al.; Formulation and quality control of semi-solid containing harmless bacteria by-products: chronic wounds pro-healing activity; Taylor & Francis; Pharmaceutical Development And Technology; 20; 8; -1-2015; 911-918
Compartir
Altmétricas