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Artículo

Electrochemical microfluidic immunosensor based on TES-AuNPs@Fe3O4 and CMK-8 for IgG anti-Toxocara canis determination

Jofre, Claudio FranciscoIcon ; Regiart, Daniel Matias GastonIcon ; Fernández Baldo, Martín AlejandroIcon ; Bertotti, Mauro; Raba, JulioIcon ; Messina, Germán AlejandroIcon
Fecha de publicación: 02/2020
Editorial: Elsevier Science
Revista: Analytica Chimica Acta
ISSN: 0003-2670
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Química Analítica

Resumen

We report a microfluidic immunosensor for the electrochemical determination of IgG antibodies anti-Toxocara canis (IgG anti-T. canis). In order to improve the selectivity and sensitivity of the sensor, core-shell gold-ferric oxide nanoparticles (AuNPs@Fe3O4), and ordered mesoporous carbon (CMK-8) in chitosan (CH) were used. IgG anti-T. canis antibodies detection was carried out using a non-competitive immunoassay, in which excretory secretory antigens from T. canis second-stage larvae (TES) were covalently immobilized on AuNPs@Fe3O4. CMK-8-CH and AuNPs@Fe3O4 were characterized by transmission electron microscopy, scanning electron microscopy, energy dispersive spectrometry, cyclic voltammetry, electrochemical impedance spectroscopy, and N2 adsorption-desorption isotherms. Antibodies present in serum samples immunologically reacted with TES, and then were quantified by using a second antibody labeled with horseradish peroxidase (HRP-anti-IgG). HRP catalyzes the reduction from H2O2 to H2O with the subsequent oxidation of catechol (H2Q) to p-benzoquinone (Q). The enzymatic product was detected electrochemically at _100 mV on a modified sputtered gold electrode. The detection limit was 0.10 ng mL−1, and the coefficients of intra- and inter-assay variation were less than 6%, with a total assay time of 20 min. As can be seen, the electrochemical immunosensor is a useful tool for in situ IgG antibodies anti-T. canis determination.
Palabras clave: AUNPS@FE3O4 , ELECTROCHEMICAL , MICROFLUIDIC IMMUNOSENSOR , TOXOCARA CANIS , TOXOCARIOSIS
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/123387
URL: https://www.sciencedirect.com/science/article/pii/S000326701931267X
DOI: http://dx.doi.org/10.1016/j.aca.2019.10.040
Colecciones
Articulos(INQUISAL)
Articulos de INST. DE QUIMICA DE SAN LUIS
Citación
Jofre, Claudio Francisco; Regiart, Daniel Matias Gaston; Fernández Baldo, Martín Alejandro; Bertotti, Mauro; Raba, Julio; et al.; Electrochemical microfluidic immunosensor based on TES-AuNPs@Fe3O4 and CMK-8 for IgG anti-Toxocara canis determination; Elsevier Science; Analytica Chimica Acta; 1096; 2-2020; 120-129
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