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dc.contributor.author
Tribelli, Paula Maria  
dc.contributor.author
Luqman, Arif  
dc.contributor.author
Nguyen, Minh Thu  
dc.contributor.author
Madlung, Johannes  
dc.contributor.author
Fan, Sook Ha  
dc.contributor.author
Macek, Boris  
dc.contributor.author
Sass, Peter  
dc.contributor.author
Bitschar, Katharina  
dc.contributor.author
Schittek, Birgit  
dc.contributor.author
Kretschmer, Dorothee  
dc.contributor.author
Götz, Friedrich  
dc.date.available
2021-01-21T14:55:29Z  
dc.date.issued
2019-09  
dc.identifier.citation
Tribelli, Paula Maria; Luqman, Arif; Nguyen, Minh Thu; Madlung, Johannes; Fan, Sook Ha; et al.; Staphylococcus aureus Lpl protein triggers human host cell invasion via activation of Hsp90 receptor; Wiley Blackwell Publishing, Inc; Cellular Microbiology (print); 22; 1; 9-2019; 1-14  
dc.identifier.issn
1462-5814  
dc.identifier.uri
http://hdl.handle.net/11336/123300  
dc.description.abstract
Staphylococcus aureus is a facultative intracellular pathogen. Recently, it has been shown that the protein part of the lipoprotein-like lipoproteins (Lpls), encoded by the lpl cluster comprising of 10 lpls paralogue genes, increases pathogenicity, delays the G2/M phase transition, and also triggers host cell invasion. Here, we show that a recombinant Lpl1 protein without the lipid moiety binds directly to the isoforms of the human heat shock proteins Hsp90α and Hsp90ß. Synthetic peptides covering the Lpl1 sequence caused a twofold to fivefold increase of S. aureus invasion in HaCaT cells. Antibodies against Hsp90 decrease S. aureus invasion in HaCaT cells and in primary human keratinocytes. Additionally, inhibition of ATPase function of Hsp90 or silencing Hsp90α expression by siRNA also decreased the S. aureus invasion in HaCaT cells. Although the Hsp90ß is constitutively expressed, the Hsp90α isoform is heat-inducible and appears to play a major role in Lpl1 interaction. Pre-incubation of HaCaT cells at 39°C increased both the Hsp90α expression and S. aureus invasion. Lpl1-Hsp90 interaction induces F-actin formation, thus, triggering an endocytosis-like internalisation. Here, we uncovered a new host cell invasion principle on the basis of Lpl-Hsp90 interaction.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Wiley Blackwell Publishing, Inc  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
F-ACTIN  
dc.subject
HSP90 RECEPTOR  
dc.subject
INVASION  
dc.subject
LPL  
dc.subject
STAPHYLOCOCCUS AUREUS  
dc.subject.classification
Biología Celular, Microbiología  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Staphylococcus aureus Lpl protein triggers human host cell invasion via activation of Hsp90 receptor  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2020-12-01T16:27:09Z  
dc.journal.volume
22  
dc.journal.number
1  
dc.journal.pagination
1-14  
dc.journal.pais
Reino Unido  
dc.journal.ciudad
Londres  
dc.description.fil
Fil: Tribelli, Paula Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Eberhard Karls Universität Tübingen.; Alemania. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina  
dc.description.fil
Fil: Luqman, Arif. Eberhard Karls Universität Tübingen.; Alemania  
dc.description.fil
Fil: Nguyen, Minh Thu. Eberhard Karls Universität Tübingen.; Alemania  
dc.description.fil
Fil: Madlung, Johannes. Eberhard Karls Universität Tübingen.; Alemania  
dc.description.fil
Fil: Fan, Sook Ha. Eberhard Karls Universität Tübingen.; Alemania  
dc.description.fil
Fil: Macek, Boris. Eberhard Karls Universität Tübingen.; Alemania  
dc.description.fil
Fil: Sass, Peter. Eberhard Karls Universität Tübingen.; Alemania  
dc.description.fil
Fil: Bitschar, Katharina. Eberhard Karls Universität Tübingen.; Alemania  
dc.description.fil
Fil: Schittek, Birgit. Eberhard Karls Universität Tübingen.; Alemania  
dc.description.fil
Fil: Kretschmer, Dorothee. Eberhard Karls Universität Tübingen.; Alemania  
dc.description.fil
Fil: Götz, Friedrich. Eberhard Karls Universität Tübingen.; Alemania  
dc.journal.title
Cellular Microbiology (print)  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/cmi.13111  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/cmi.13111