Artículo
Silencing of the transcription factors Oct4, Sox2, Klf4, c-Myc or Nanog has different effect on teratoma growth
Villodre, Emilly Schlee; Felipe, Karina Bettega; Oyama, Mayumi Zanotta; de Oliveira, Francine Hehn; Lopez, Patrícia Luciana da Costa; Solari, Claudia María
; Sevlever, Gustavo; Guberman, Alejandra Sonia
; Lenz, G.
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
07/2019
Editorial:
Academic Press Inc Elsevier Science
Revista:
Biochemical and Biophysical Research Communications
ISSN:
0006-291X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Induced pluripotent stem cells (iPSC) have a great potential, but their clinical application depends on finding strategies to abolish their tumorigenic potential. The use of Oct4, Sox2, Klf4, c-Myc and Nanog to generate iPSC demonstrated the already known importance of these genes to maintain stemness. Therefore, the presence of these genes is responsible for iPSC-derived teratomas. Similar to iPSC, P19 teratocarcinoma cell line also has characteristics of embryonic carcinoma cells and the ability to differentiate into many cell types. We separately silenced the transcription factors Oct4, Sox2, Klf4, c-Myc and Nanog in P19 cells and measured the impact of this silencing in vivo. All silenced cells generated tumors when injected in immunosuppressed mice, but silencing of Oct4, Sox2 and Klf4 generated mainly teratomas with mesoderm tissue. Our results suggest that downregulation of these transcription factors is not enough to avoid the formation of teratomas, but their silencing affect their differentiation potential.
Palabras clave:
EMBRYONIC STEM CELL
,
KNOCKDOWN
,
P19 CELL LINE
,
TERATOCARCINOMA
,
YAMANAKA FACTORS
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Articulos(IQUIBICEN)
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Citación
Villodre, Emilly Schlee; Felipe, Karina Bettega; Oyama, Mayumi Zanotta; de Oliveira, Francine Hehn; Lopez, Patrícia Luciana da Costa; et al.; Silencing of the transcription factors Oct4, Sox2, Klf4, c-Myc or Nanog has different effect on teratoma growth; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 517; 2; 7-2019; 324-329
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