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dc.contributor.author
Sander, Valeria Analía
dc.contributor.author
Facorro, Graciela
dc.contributor.author
Piehl, Lidia Leonor
dc.contributor.author
Rubin de Cellis, E
dc.contributor.author
Motta, Alicia Beatriz
dc.date.available
2021-01-20T17:38:26Z
dc.date.issued
2009-09
dc.identifier.citation
Sander, Valeria Analía; Facorro, Graciela; Piehl, Lidia Leonor; Rubin de Cellis, E; Motta, Alicia Beatriz; Effect of DHEA and metformin on corpus luteum in mice; BioScientifica; Reproduction; 138; 3; 9-2009; 571-579
dc.identifier.issn
1470-1626
dc.identifier.uri
http://hdl.handle.net/11336/123214
dc.description.abstract
Effect of DHEA and metformin on corpus luteum in mice Abstract We evaluated the effect of hyperandrogenism in ovaries with functional and regressing corpora lutea (CL) and the action of metformin in preventing these possible alterations using a mouse model. To obtain a CL functional for 9+/-1 days, immature female mice of the BALB/c strain were injected i.p. with 10 IU/mouse of pregnant mare´s serum gonadotropin (PMSG). DHEA (60 mg/kg body weight s.c., 24 and 48 h prior to kill) decreased both serum progesterone (P) and estradiol (E(2)) levels and increased the activity of superoxide dismutase (SOD) from ovaries with functional CL (on day 5 after PMSG). It increased P and E(2) and the activities of SOD and catalase (CAT) and decreased lipoperoxidation of ovaries with regressing CL (on day 9 after PMSG). Treatment with DHEA did not affect the production of prostaglandin F(2alpha) (PGF(2alpha)) or PGE by ovaries with functional CL, whereas DHEA decreased PGF(2alpha) and increased PGE production by ovaries with regressing CL. Metformin (50 mg/kg body weight, orally) given together with DHEA restored E(2) levels from mice with ovaries with functional CL and serum P, PGF(2alpha) and PGE levels, and oxidative balance in mice with ovaries with regressing CL. Metformin alone was able to modulate serum P and E(2) levels, lipoperoxidation, SOD and CAT, and the 5,5-dimethyl-1-pyrroline N-oxide/(*)OH signal. These findings suggest that hyperandrogenism is able to induce or to rescue CL from luteolysis and metformin treatment is able to prevent these effects.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
BioScientifica
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
DHEA
dc.subject
Metformina
dc.subject
Corpus luteum
dc.subject
oxidative stress
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Farmacología y Farmacia
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Effect of DHEA and metformin on corpus luteum in mice
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-09-11T19:45:39Z
dc.journal.volume
138
dc.journal.number
3
dc.journal.pagination
571-579
dc.journal.pais
Reino Unido
dc.journal.ciudad
Woodlands
dc.description.fil
Fil: Sander, Valeria Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas (subsede Chascomús) | Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas (subsede Chascomús); Argentina
dc.description.fil
Fil: Facorro, Graciela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Físico Matemática; Argentina
dc.description.fil
Fil: Piehl, Lidia Leonor. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Físico Matemática; Argentina
dc.description.fil
Fil: Rubin de Cellis, E. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Fisicomatemática. Cátedra de Física; Argentina
dc.description.fil
Fil: Motta, Alicia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
dc.journal.title
Reproduction
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/138/3/571.short
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1530/REP-08-0325
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