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dc.contributor.author
Jeong, Yeon Tae
dc.contributor.author
Rossi, Mario
dc.contributor.author
Cermak, Lukas
dc.contributor.author
Saraf, Anita
dc.contributor.author
Florens, Laurence
dc.contributor.author
Washburn, Michael P.
dc.contributor.author
Sung, Patrick
dc.contributor.author
Schildkraut, Carl
dc.contributor.author
Pagano, Michele
dc.date.available
2017-02-01T15:42:05Z
dc.date.issued
2013-01
dc.identifier.citation
Jeong, Yeon Tae; Rossi, Mario; Cermak, Lukas; Saraf, Anita; Florens, Laurence; et al.; FBH1 mediates DNA double strand breakage and apoptosis in response to DNA replication stress; Rockefeller Univ Press; Journal Of Cell Biology; 200; 2; 1-2013; 141-149
dc.identifier.issn
0021-9525
dc.identifier.uri
http://hdl.handle.net/11336/12304
dc.description.abstract
Proper resolution of stalled replication forks is essential for genome stability. Purification of FBH1, a UvrD DNA helicase, identified a physical interaction with replication protein A (RPA), the major cellular singlestranded DNA (ssDNA)?binding protein complex. Compared with control cells, FBH1-depleted cells responded to replication stress with considerably fewer double-strand breaks (DSBs), a dramatic reduction in the activation of<br />ATM and DNA-PK and phosphorylation of RPA2 and p53, and a significantly increased rate of survival. A minor decrease in ssDNA levels was also observed. All these phenotypes were rescued by wild-type FBH1, but not a FBH1 mutant lacking helicase activity. FBH1 depletion had no effect on other forms of genotoxic stress in which DSBs form by means that do not require ssDNA intermediates. In response to catastrophic genotoxic stress, apoptosis prevents the persistence and propagation of DNA lesions. Our findings show that FBH1 helicase activity is required for the efficient induction of DSBs and apoptosis specifically in response to DNA replication stress.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Rockefeller Univ Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Ubiquitin
dc.subject
Replication
dc.subject
Hydroxyurea
dc.subject
Replication Protein A
dc.subject.classification
Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
FBH1 mediates DNA double strand breakage and apoptosis in response to DNA replication stress
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-12-12T20:46:35Z
dc.journal.volume
200
dc.journal.number
2
dc.journal.pagination
141-149
dc.journal.pais
Estados Unidos
dc.journal.ciudad
New York
dc.description.fil
Fil: Jeong, Yeon Tae. University Of New York; Estados Unidos
dc.description.fil
Fil: Rossi, Mario. University Of New York; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires; Argentina
dc.description.fil
Fil: Cermak, Lukas. University Of New York; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos
dc.description.fil
Fil: Saraf, Anita. The Stowers Institute for Medical Research; Estados Unidos
dc.description.fil
Fil: Florens, Laurence. The Stowers Institute for Medical Research; Estados Unidos
dc.description.fil
Fil: Washburn, Michael P.. The Stowers Institute for Medical Research; Estados Unidos. University of Kansas; Estados Unidos
dc.description.fil
Fil: Sung, Patrick. University Of Yale; Estados Unidos
dc.description.fil
Fil: Schildkraut, Carl. Albert Einstein College of Medicine; Estados Unidos
dc.description.fil
Fil: Pagano, Michele. University Of New York; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos
dc.journal.title
Journal Of Cell Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://jcb.rupress.org/content/200/2/141.long
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3549964/
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1083/jcb.201209002
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