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Artículo

Antimicrobial activity of bacteriocin-producing Carnobacterium spp. isolated from healthy Patagonian trout and their potential for use in aquaculture

Garcés, Marisa ElisabethIcon ; Olivera, Nelda LilaIcon ; Fernández, MelaniaIcon ; Riva Rossi, Carla MarcelaIcon ; Sequeiros, CynthiaIcon
Fecha de publicación: 11/2020
Editorial: Wiley Blackwell Publishing, Inc
Revista: Aquaculture Research
ISSN: 1355-557X
e-ISSN: 1365-2109
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Celular, Microbiología

Resumen

In this study, bacteria from the intestines of Patagonian trout displaying fish pathogen antimicrobial activity were isolated and characterized. Isolates T4, T15 and M5 showed extracellular antimicrobial activity against the fish pathogen Carnobacterium maltaromaticum CECT 4020, by the agar well diffusion assay. They were identified as Gram-positive catalase-negative bacilli belonging to the genus Carnobacterium (based on 99.93% identity with the 16S rRNA gene sequence of C. maltaromaticum DSM 20342T). After 48 hr of incubation in MRS broth, the highest antimicrobial activity titre (327,680 AU/ml) was recorded for T4, followed by T15 and M5. The antimicrobial agents produced by these isolates were thermostable peptides. LC-MS/MS peptide analysis indicated that T4 and T5 bacteriocins belonged to the class IIa. The identified peptides for T4 bacteriocin included almost the entire divercin V41 sequence, which was previously believed to be exclusively produced by Carnobacterium divergens. In addition, no negative fish health effects were observed after administrating T4 to zebrafish. Among the isolates from healthy Patagonian trout, due to its early bacteriocin production, its greater inhibition titre and its innocuous feature, Carnobacterium sp. T4 emerged as the most promising isolate for in vivo challenges targeting its further aquaculture application.
Palabras clave: ANTIMICROBIAL ACTIVITY , AQUACULTURE , CARNOBACTERIUM MALTAROMATICUM , DIVERCIN V41 , LACTIC ACID BACTERIA , RAINBOW TROUT
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/122716
DOI: http://dx.doi.org/10.1111/are.14806
URL: https://onlinelibrary.wiley.com/doi/10.1111/are.14806
Colecciones
Articulos(IDEAUS)
Articulos de INSTITUTO DE DIVERSIDAD Y EVOLUCION AUSTRAL
Citación
Garcés, Marisa Elisabeth; Olivera, Nelda Lila; Fernández, Melania; Riva Rossi, Carla Marcela; Sequeiros, Cynthia; Antimicrobial activity of bacteriocin-producing Carnobacterium spp. isolated from healthy Patagonian trout and their potential for use in aquaculture; Wiley Blackwell Publishing, Inc; Aquaculture Research; 51; 11; 11-2020; 4602-4612
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