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dc.contributor.author
Garro, María Laura
dc.contributor.author
Galarza, Betina Claudia
dc.contributor.author
Greco, Carlos A.
dc.contributor.author
Hours, Roque Alberto
dc.date.available
2021-01-14T12:24:14Z
dc.date.issued
2019-02
dc.identifier.citation
Garro, María Laura; Galarza, Betina Claudia; Greco, Carlos A.; Hours, Roque Alberto; Unhairing of bovine skin with fungal enzymes by immersion and spread throughout the epidermis; Society of Leather Technologists and Chemists; Journal Of The Society Of Leather Technologists And Chemists; 103; 2-2019; 28-34
dc.identifier.issn
0144-0322
dc.identifier.uri
http://hdl.handle.net/11336/122707
dc.description.abstract
The use of enzymes during unhairing in the leather industry to partially reduce sulfide can decrease H2S emission to almost 50%. However the proteolysis needs to be controlled to prevent collagen damage. In bovine epidermis, the stratum corneum plays a crucial role as a hydrophobic barrier. In order to facilitate the diffusion of enzymes, it is necessary to generate channels through this barrier to allow enzymes pass without damaging the collagen. In an ideal enzymatic unhairing the proteolytic activity should be restricted to the basal lamina and pilosebaceous unit. During this experiment, fungal enzyme extracts (CE) were applied on bovine skin in the soaking and unhairing steps as part of the tanning process. The skin contacted with the solutions in two different ways: in direct contact with the epidermis (Mode I) and immersed in the solution (Mode II). Various keratinolytic fungi, previously isolated and selected from different soil samples were used: Neurospora crassa, Verticillium albo-atrum, Trichophyton ajelloi, Chrysosporium sp, Aspergillus sydowii, Paecilomyces lilacinus and Acremonium murorum. The fungal isolates were cultivated under solid state conditions using hair waste obtained from the hair-saving unhairing process as substrate. Enzyme extracts were characterized according to their keratinolytic and proteolytic activity and protein content. Once the immersion with fungal enzymes was finished, the treatment with N. crassa, T. ajelloi, Chrysosporium sp, A. sydowii and P. lilacinus, showed unhaired skin and epidermis removed. After the contact between the epidermis and fungal enzymatic extract of T. ajelloi empty hair follicles were observed. The morphological changes were studied with SEM (Scanning Electron Microscopy).
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Society of Leather Technologists and Chemists
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
UNHAIRING
dc.subject
BOVINE SKIN
dc.subject
FUNGAL ENZYMES
dc.subject
SEM
dc.subject.classification
Bioproductos, Biomateriales, Bioplásticos, Biocombustibles, Bioderivados, etc.
dc.subject.classification
Biotecnología Industrial
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
dc.title
Unhairing of bovine skin with fungal enzymes by immersion and spread throughout the epidermis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-11-25T18:00:46Z
dc.journal.volume
103
dc.journal.pagination
28-34
dc.journal.pais
Reino Unido
dc.journal.ciudad
West Yorkshire
dc.description.fil
Fil: Garro, María Laura. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo del Cuero. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Centíficas. Centro de Investigación y Desarrollo del Cuero; Argentina
dc.description.fil
Fil: Galarza, Betina Claudia. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo del Cuero. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Centíficas. Centro de Investigación y Desarrollo del Cuero; Argentina
dc.description.fil
Fil: Greco, Carlos A.. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo del Cuero. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Centíficas. Centro de Investigación y Desarrollo del Cuero; Argentina
dc.description.fil
Fil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
dc.journal.title
Journal Of The Society Of Leather Technologists And Chemists
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