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dc.contributor.author
Núñez Aguilera, Felipe Javier
dc.contributor.author
Mendez, Flor M.
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Garcia Fabbiani, María B.
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Pardo, Joaquin
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Edwards, Marta
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Lowenstein, Pedro R.
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Castro, María G.
dc.date.available
2021-01-13T21:21:39Z
dc.date.issued
2019-01-09
dc.identifier.citation
Núñez Aguilera, Felipe Javier; Mendez, Flor M.; Garcia Fabbiani, María B.; Pardo, Joaquin; Edwards, Marta; et al.; Evaluation of biomarkers in glioma by immunohistochemistry on paraffin- embedded 3D glioma neurosphere cultures; Journal of Visualized Experiments; Journal of Visualized Experiments; 2019; 143; 9-1-2019; 1-7
dc.identifier.issn
1940-087X
dc.identifier.uri
http://hdl.handle.net/11336/122684
dc.description.abstract
Analysis of protein expression in glioma is relevant for several aspects in the study of its pathology. Numerous proteins have been described asbiomarkers with applications in diagnosis, prognosis, classification, state of tumor progression, and cell differentiation state. These analyses ofbiomarkers are also useful to characterize tumor neurospheres (NS) generated from glioma patients and glioma models. Tumor NS provide avaluable in vitro model to assess different features of the tumor from which they are derived and can more accurately mirror glioma biology. Herewe describe a detailed method to analyze biomarkers in tumor NS using immunohistochemistry (IHC) on paraffin-embedded tumor NS.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Journal of Visualized Experiments
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
3D-CULTURES
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BIOMARKERS
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CANCER RESEARCH
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GENETICALLY ENGINEERED
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GLIOMA
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IMMUNOSTAINING
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ISSUE 143
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NEUROSPHERES
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PARAFFIN EMBEDDING
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Evaluation of biomarkers in glioma by immunohistochemistry on paraffin- embedded 3D glioma neurosphere cultures
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-11-25T17:35:41Z
dc.journal.volume
2019
dc.journal.number
143
dc.journal.pagination
1-7
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Núñez Aguilera, Felipe Javier. University of Michigan; Estados Unidos
dc.description.fil
Fil: Mendez, Flor M.. University of Michigan; Estados Unidos
dc.description.fil
Fil: Garcia Fabbiani, María B.. University of Michigan; Estados Unidos
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Fil: Pardo, Joaquin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina. University of Michigan; Estados Unidos
dc.description.fil
Fil: Edwards, Marta. University of Michigan; Estados Unidos
dc.description.fil
Fil: Lowenstein, Pedro R.. University of Michigan; Estados Unidos
dc.description.fil
Fil: Castro, María G.. University of Michigan; Estados Unidos
dc.journal.title
Journal of Visualized Experiments
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.jove.com/video/58931/evaluation-biomarkers-glioma-immunohistochemistry-on-paraffin
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3791/58931
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