Artículo
Highly efficient production of rabies virus glycoprotein G ectodomain in Sf9 insect cells
Targovnik, Alexandra Marisa
; Ferrari, Alejandro
; Mc Callum, Gregorio Juan
; Arregui, Mariana Bernadett
; Smith, Ignacio
; Bracco, Lautaro Fidel
; Alfonso, Victoria
; López, María Gabriela
; Martínez Solís, María; Herrero, Salvador; Miranda, María Victoria
Fecha de publicación:
10/2019
Editorial:
Springer
Revista:
3 Biotech
ISSN:
2190-572X
e-ISSN:
2190-5738
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
In the present study, we developed a complete process to produce in insect cells a high amount of the ectodomain of rabies virus glycoprotein G (GE) as suitable antigen for detecting anti-rabies antibodies. Using the baculovirus expression vector system in Sf9 insect cells combined with a novel chimeric promoter (polh-pSeL), the expression level reached a yield of 4.1± 0.3 mg/L culture, which was signifcantly higher than that achieved with the standard polh promoter alone. The protein was recovered from the cell lysates and easily purifed in only one step by metal ion afnity chromatography, with a yield of 95% and a purity of 87%. Finally, GE was successfully used in an assay to detect specifc antibodies in serum samplesderived from rabies-vaccinated animals. The efcient strategy developed in this work is an interesting method to produce high amounts of this glycoprotein.
Palabras clave:
BACULOVIRUS
,
RABIES VIRUS GLYCOPROTEIN
,
POLH PSEL PROMOTER
,
SF9 CELLS
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Colecciones
Articulos(IQUIFIB)
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Articulos(NANOBIOTEC)
Articulos de INSTITUTO DE NANOBIOTECNOLOGIA
Articulos de INSTITUTO DE NANOBIOTECNOLOGIA
Citación
Targovnik, Alexandra Marisa; Ferrari, Alejandro; Mc Callum, Gregorio Juan; Arregui, Mariana Bernadett; Smith, Ignacio; et al.; Highly efficient production of rabies virus glycoprotein G ectodomain in Sf9 insect cells; Springer; 3 Biotech; 9; 11; 10-2019; 1-11
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