Artículo
Obtaining an Ent35-MccV derivative with mutated hinge region that exhibits increased activity against listeria monocytogenes and escherichia coli
Navarro, Silvia Adriana
; Lanza, Lucía
; Ríos Colombo, Natalia Soledad
; Fernandez de Ullivarri, Miguel
; Acuña, Leonardo
; Sosa Padilla Araujo, Bernardo
; Picariello, Gianluca; Bellomio, Augusto
; Chalon, Miriam Carolina
Fecha de publicación:
11/2019
Editorial:
Springer
Revista:
Applied Microbiology and Biotechnology
ISSN:
0175-7598
e-ISSN:
1432-0614
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The present paper describes the generation of derivatives from the hybrid peptide called Ent35-MccV, active against Gram-positive and Gram-negative bacteria. This peptide has a triple glycine hinge region between enterocin CRL35 and microcin V. In order to obtain variants of Ent35-MccV with greater biotechnological potential, a saturation mutagenesis was carried out in the hinge region. As a result, we obtained a bank of E.coli strains expressing different mutated hybrid bacteriocins in the central position of the hinge region. From all these variants, we found that the one bearing a tyrosine in the central region of the hinge (Ent35-GYG-MccV) is 2-fold more active against E. coli and 4-fold more active against Listeria than the original peptide Ent35-MccV. This derivative was purified and characterized. The development and evaluation of alternative hinges for Ent35-MccV represents a step forward in the bioengineering of antimicrobial peptides. This approach fosters the rational design of peptides with enhanced antimicrobial activity.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(INSIBIO)
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Articulos(IPE)
Articulos de INST.DE PATOLOGIA EXPERIMENTAL
Articulos de INST.DE PATOLOGIA EXPERIMENTAL
Articulos(PROIMI)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Citación
Navarro, Silvia Adriana; Lanza, Lucía; Ríos Colombo, Natalia Soledad; Fernandez de Ullivarri, Miguel; Acuña, Leonardo; et al.; Obtaining an Ent35-MccV derivative with mutated hinge region that exhibits increased activity against listeria monocytogenes and escherichia coli; Springer; Applied Microbiology and Biotechnology; 103; 23-24; 11-2019; 9607-9618
Compartir
Altmétricas