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dc.contributor.author
Poyato Bonilla, Julia
dc.contributor.author
Anaya Calvo, Gabriel
dc.contributor.author
Molina, Antonio
dc.contributor.author
Valera, Mercedes
dc.contributor.author
Moreno Millán, Miguel
dc.contributor.author
Dorado, Jesús
dc.contributor.author
Demyda Peyrás, Sebastián
dc.date.available
2021-01-05T02:36:31Z
dc.date.issued
2019-03
dc.identifier.citation
Poyato Bonilla, Julia; Anaya Calvo, Gabriel; Molina, Antonio; Valera, Mercedes; Moreno Millán, Miguel; et al.; A new molecular screening tool for the detection of chromosomal abnormalities in donkeys; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 54; 3; 3-2019; 580-584
dc.identifier.issn
0936-6768
dc.identifier.uri
http://hdl.handle.net/11336/121442
dc.description.abstract
Chromosomal abnormalities are a major cause of infertility and reproductive problems in equids. Nowadays, their detection is rising due to the use of new diagnostic tools based on molecular markers instead of karyotyping. Reports of this kind of genetic aberrations in domestic donkeys (Equus asinus) are extremely scarce, despite their importance in human activities. In the present study, we analysed the implementation of a short-tandem-repeat (STR)-based molecular method initially developed for horses, as a diagnostic tool to detect chromosomal abnormalities in donkeys. The frequency of five X-linked (LEX003, LEX026, TKY38, TKY270 and UCEDQ502) and one Y-linked (ECAYM2) molecular markers and one Y-linked gene (sex-determining region Y, SRY) was characterized in 121 donkeys from two diverse breeds, the Spanish Andalusian and the African Moroccan breeds. The molecular panel showed 100% sensitivity and 99.67% specificity in detecting 10 different chromosomal abnormalities in the species. In conclusion, this methodology is a valid, rapid and low-cost tool for the detection and characterization of chromosomal abnormalities in domestic donkeys.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CHROMOSOMAL ABERRATIONS
dc.subject
CHROMOSOMOPATHIES
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DIAGNOSTIC TOOL
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EQUUS ASINUS
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MICROSATELLITES
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MOLECULAR CHARACTERIZATION
dc.subject.classification
Tecnología GM, clonación de ganado, selección asistida, diagnósticos, tecnología de producción de biomasa, etc.
dc.subject.classification
Biotecnología Agropecuaria
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
A new molecular screening tool for the detection of chromosomal abnormalities in donkeys
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-11-18T16:20:12Z
dc.identifier.eissn
1439-0531
dc.journal.volume
54
dc.journal.number
3
dc.journal.pagination
580-584
dc.journal.pais
Reino Unido
dc.description.fil
Fil: Poyato Bonilla, Julia. Universidad de Sevilla; España
dc.description.fil
Fil: Anaya Calvo, Gabriel. Universidad de Córdoba; España
dc.description.fil
Fil: Molina, Antonio. Universidad de Córdoba; España
dc.description.fil
Fil: Valera, Mercedes. Universidad de Sevilla; España
dc.description.fil
Fil: Moreno Millán, Miguel. Universidad de Córdoba; España
dc.description.fil
Fil: Dorado, Jesús. Universidad de Córdoba; España
dc.description.fil
Fil: Demyda Peyrás, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
dc.journal.title
Reproduction in Domestic Animals
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://doi.wiley.com/10.1111/rda.13398
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/rda.13398
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