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dc.contributor.author
Byrum, Stephanie D.
dc.contributor.author
Washam, Charity L.
dc.contributor.author
Tackett, Alan J.
dc.contributor.author
Garcia Rill, Edgar
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dc.contributor.author
Bisagno, Veronica
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dc.contributor.author
Urbano Suarez, Francisco Jose
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dc.date.available
2020-12-28T16:53:45Z
dc.date.issued
2019-08
dc.identifier.citation
Byrum, Stephanie D.; Washam, Charity L.; Tackett, Alan J.; Garcia Rill, Edgar; Bisagno, Veronica; et al.; Proteomic measures of gamma oscillations; Elsevier; Heliyon; 5; 8; 8-2019; 2265-2272
dc.identifier.issn
2405-8440
dc.identifier.uri
http://hdl.handle.net/11336/121236
dc.description.abstract
Background: Gamma oscillations serve complex processes, and the first stage of their generation is the reticular activating system (RAS), which mediates the gamma-activity states of waking and paradoxical sleep. We studied whether the pedunculopontine nucleus (PPN), part of the RAS in which every cell manifests intrinsic gamma oscillations, undergoes changes resulting in distinctive protein expression. New method: We previously found that a histone deacetylation inhibitor, trichostatin A (TSA), acutely (30 min) blocked these oscillations. We developed a proteomic method for sampling stimulated and unstimulated PPN and determining protein expression in 1 mm punches of tissue from brain slices subjected to various treatments. Results: We compared brain slices exposed for 30 min to TSA (unstimulated), to the cholinergic agonist carbachol (CAR), known to induce PPN gamma oscillations, or exposed to both TSA + CAR. Comparison with existing methods: Label-free proteomics provides an unbiased and sensitive method to detect protein changes in the PPN. Our approach is superior to antibody-based methods that can lack specificity and can only be done for known targets. Proteomics methods like these have been leveraged to study molecular pathways in numerous systems and disease states. Conclusions: Significant protein changes were seen in two functions essential to the physiology of the PPN: cytoskeletal and intracellular [Ca2+] regulation proteins. TSA decreased, while CAR increased, and TSA + CAR had intermediate effects, on expression of these proteins. These results support the feasibility of the methods developed for determining proteomic changes in small samples of tissue participating in the most complex of brain processes.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
NEUROSCIENCE
dc.subject.classification
Neurociencias
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Proteomic measures of gamma oscillations
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-11-13T20:45:18Z
dc.journal.volume
5
dc.journal.number
8
dc.journal.pagination
2265-2272
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Londres
dc.description.fil
Fil: Byrum, Stephanie D.. Arkansas Children's Research Institute; Estados Unidos
dc.description.fil
Fil: Washam, Charity L.. Arkansas Children's Research Institute; Estados Unidos
dc.description.fil
Fil: Tackett, Alan J.. Arkansas Children's Research Institute; Estados Unidos
dc.description.fil
Fil: Garcia Rill, Edgar. University of Arkansas for Medical Sciences; Estados Unidos
dc.description.fil
Fil: Bisagno, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; Argentina
dc.description.fil
Fil: Urbano Suarez, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
dc.journal.title
Heliyon
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.heliyon.2019.e02265
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