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Artículo

Global transcriptome analysis reveals circadian control of splicing events in Arabidopsis thaliana

Romanowski, AndrésIcon ; Schlaen, Rubén GustavoIcon ; Perez Santangelo, Maria SoledadIcon ; Mancini, EstefaniaIcon ; Yanovsky, Marcelo JavierIcon
Fecha de publicación: 05/2020
Editorial: Wiley Blackwell Publishing, Inc
Revista: Plant Journal
ISSN: 0960-7412
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología

Resumen

The circadian clock of Arabidopsis thaliana controls many physiological and molecular processes, allowing plants to anticipate daily changes in their environment. However, developing a detailed understanding of how oscillations in mRNA levels are connected to oscillations in co/post-transcriptional processes, such as splicing, has remained a challenge. Here we applied a combined approach using deep transcriptome sequencing and bioinformatics tools to identify novel circadian-regulated genes and splicing events. Using a stringent approach, we identified 300 intron retention, eight exon skipping, 79 alternative 3' splice site usage, 48 alternative 5' splice site usage, and 350 multiple (more than one event type) annotated events under circadian regulation. We also found seven and 721 novel alternative exonic and intronic events. Depletion of the circadian-regulated splicing factor AtSPF30 homologue resulted in the disruption of a subset of clock-controlled splicing events. Altogether, our global circadian RNA-seq coupled with an in silico, event-centred, splicing analysis tool offers a new approach for studying the interplay between the circadian clock and the splicing machinery at a global scale. The identification of many circadian-regulated splicing events broadens our current understanding of the level of control that the circadian clock has over this co/post-transcriptional regulatory layer.
Palabras clave: ALTERNATIVE SPLICING , ARABIDOPSIS THALIANA , CIRCADIAN CLOCK , CIRCADIAN RHYTHMS , SPF30 , SPLICING FACTORS
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/120995
URL: https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.14776
DOI: http://dx.doi.org/10.1111/tpj.14776
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Articulos(IIBBA)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Citación
Romanowski, Andrés; Schlaen, Rubén Gustavo; Perez Santangelo, Maria Soledad; Mancini, Estefania; Yanovsky, Marcelo Javier; Global transcriptome analysis reveals circadian control of splicing events in Arabidopsis thaliana; Wiley Blackwell Publishing, Inc; Plant Journal; 103; 2; 5-2020; 889-902
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